Novel cloning vehicles for polypeptide expression in microbial h

Details Novel cloning vehicles for polypeptide expression in microbial h Novel cloning vehicles for polypeptide expression in microbial h

1982-03-03

1986-11-25

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

4351723, 935 29, 935 41, 935 48, 935 73, C12N 120, C12N 1500, C12N 100

Patent

active

046249265

ABSTRACT:
Methods and compositions are provided for expression of polypeptides in transformed bacterial hosts. A novel class of plasmid cloning vehicles includes a DNA sequence coding for the desired polypeptide linked in reading phase with one or more functional fragments derived from an outer membrane protein gene of a gramnegative bacterium. The methods utilize such plasmids to introduce genetic capability into micro-organisms for the production of proteins, such as medically or commercially useful hormones, enzymes, immunogenic proteins, or intermediates therefor.

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