Novel cloning vectors containing selectable genetic markers for

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of...

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435 68, 435 70, 435 71, 435 91, 4351723, 435317, 435253, 536 27, 935 6, 935 29, 935 75, C12N 100, C12N 1500, C12N 120, C12P 2100, C12P 2102, C12P 2104, C12P 1934, C07H 2104

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046668462

ABSTRACT:
The present invention discloses novel recombinant DNA cloning vectors for use in Streptomyces and related organisms. These novel cloning vectors contain genetic markers that provide antibiotic resistance or colorimetric selectivity to the host cells. The invention further comprises transformants of the aforementioned vectors.

REFERENCES:
patent: 4416994 (1983-11-01), Nakatsukasa et al.
Jones et al, "pFJ269, A New Plasmid Isolated from a Beta-Lactam Antibiotic Producing Streptomycete", Chem. Abstr., 102:56905r (1985) of J. Antibiotic. 37(12), 1727, Dec. (1984).
Soberon et al, "Construction and Characterization of New Cloning Vehicles., IV., Deletion Derivatives of pBR322 and pBR325", Chem. Abstr., 93:65765t (1980) of Gene 9:287 (1980).
American Type Culture Collection Catalogue of Strains I, Fifteenth Edition, 1982, p. 259.
Thompson, C. et al., 1980 Nature 286:525.
Hopwood, D. A. et al., 1983, Trends in Biotechnology, 1:42.
Katz, E. et al., 1983, J. Gen. Microbiol., 129:1703.
Richardson, M. et al., 1982, Gene 20:451.

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