Novel biologically-active compounds and method for isolating the

Details Novel biologically-active compounds and method for isolating the Novel biologically-active compounds and method for isolating the

1974-01-22

1976-03-16

Waddell, Frederick E.

Drug, bio-affecting and body treating compositions

Fermentate of unknown chemical structure

Having a known elemental analysis

424110, A61K 2700

Patent

active

039446654

ABSTRACT:
The biologically-active compounds disclosed herein are two low-molecular water-soluble compounds that are formed in the incretory apparatus of the pancreas, which exhibit a coronary dilating effect and which are characterized by the following physico-chemical and biological properties, on chromatograms with a butyl alcohol-acetic acid-water (4:1:5) solvent system, the distribution coefficient Rf for these compounds is 0.45 and 0.6, respectively; in an electrophoretic field of 1500 v, at a pH of 3.8, an ionic strength of 0.1 and a current density of 1.2 mA/cm, one of these compounds moves toward the cathode (-6 cm) and the other towards the anode (+4 cm); they may be dialyzed through a semi-permeable membrane; they do not affect the glucose level of blood during their intravenous administration; the molecular weights according to the chromatographic, electrophoretic and gel filtration data is from 300-400 and from 200-300, respectively.
The method for isolating the said compounds from insulin-containing raw materials consists in that the serum of the venous blood of the pancreas is dialyzed against a 2N solution of acetic acid at a temperature of 3.degree.-10.degree.C for from 24-48 hours, the dialyzate is lyophilized, and the end products are finally isolated from the dialyzate by gel filtration, chromatography and electrophoresis.

REFERENCES:
patent: 2424401 (1947-07-01), Lesuk
patent: 2784142 (1957-03-01), Schultz

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