Novel bilirubin oxidase which has a substrate specifity to bilir

Details Novel bilirubin oxidase which has a substrate specifity to bilir Novel bilirubin oxidase which has a substrate specifity to bilir

1988-06-10

1991-01-15

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 25, 435254, 435933, C12N 902, C12N 100, C12N 114, C12Q 126

Patent

active

049853600

ABSTRACT:
A novel bilirubin oxidase is provided having a substrate specificity for bilirubin but not to, at least, biliverdin, catechol and hemin and which catalyzes the enzyme reaction between two moles of bilirubin and one mole of oxygen to yield two moles of biliverdin and two moles of water. A process is also provided for the production of said novel bilirubin oxidase, which comprises cultivating a bilirubin oxidase-producing fungus of the genus Penicillium in a culture medium and collecting the bilirubin oxidase from the culture mixture. A method of determining the content of bilirubin in a sample to be analyzed is further provided which comprises contacting the sample with the bilirubin oxidase to convert bilirubin in the sample into biliverdin and determining the amount of biliverdin formed or the amount of oxygen consumed therefor, as well as a method for removing bilirubin contained in a sample to be analyzed, which comprises contacting the sample with said novel bilirubin oxidase.

REFERENCES:
patent: 4211844 (1980-07-01), Wu
patent: 4544249 (1985-11-01), Kosaka et al.
patent: 4569912 (1986-02-01), Matsui et al.
patent: 4571383 (1986-02-01), Takayama et al.
patent: 4600689 (1986-07-01), Matsui et al.
patent: 4677062 (1987-06-01), Uwajima et al.
patent: 4770997 (1988-09-01), Yoshino et al.
"Purification and Characterization of Bacillus coagulans oligo-1,6-glucosidase", European Journal of Biochemistry, vol. 158, 1986, by Y. Suzuki et al., p. 77-83.

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