Novel assays for interferon

Details Novel assays for interferon Novel assays for interferon

1980-04-11

1981-11-24

Castel, Benoit

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 6, 435 15, 435 29, 435811, 424 85, 23230B, C12Q 102, C12Q 168

Patent

active

043025331

ABSTRACT:
The present invention relates to an assay for the quantitative determination of interferon which comprises extracting a cell previously exposed to said interferon with an extractant, and determining in such extract the quantity of an enzyme the content of which in said cell is a function of the quantity of interferon to which said cell had been previously exposed and to a kit for carrying out such assay.

REFERENCES:
patent: 3910824 (1975-10-01), Cartwright et al.
patent: 4061538 (1977-12-01), Dorner et al.
Revel, M. "Molecular Mechanisms Involved in the Antiviral Effects of Interferon", Interferon 1 1979, Grosser, I., Editor-In-Chief, Academic Press, pp. 14-151.
Kimchi, A. et al. "Kinetics of the Induction of Three Translation Regulatory Enzymes by Interferon", Proc. Natl. Acad. Sci. USA, vol. 76, pp. 3208-3212, Jul. 1979.
Zilberstein A. et al. "Specific Phosphorylation in Vitro of a Protein Associated with Ribosomes of Interferon-Treated Mouse Cells" FEBS Letters, vol. 68 #1, Sep. 1976 North-Holland Publishing Co.-Amsterdam.
Zilberstein, A. et al. "Isolation of two Interferon-Induced Translational Inhibitors: A protein Kinase and an Oligo-Isoadenylate Synthetase," Proc. Natl. Sci. USA, vol. 75, #10, pp. 4734-4738, Oct. 1978.

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