Novel amino acid substituted 4-aminophenazones for measuring enz

Details Novel amino acid substituted 4-aminophenazones for measuring enz Novel amino acid substituted 4-aminophenazones for measuring enz

1990-09-24

1992-01-14

Wax, Robert A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Heterocyclic carbon compounds containing a hetero ring...

435 15, 435 24, 435188, 436501, 530300, 548373, C07D23100

Patent

active

050812599

ABSTRACT:
Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4-aminophenazone reacts with the enzyme to produce 4-aminophenazone which is an amino dye intermediate. The amino dye intermediate is coupled with a second dye intermediate to form a chromogen. The 4-aminophenazone is particularly reacted with a phenolic naphtholic or aniline compound, particularly the phenolic compound 2-hydroxy-3,5-dichlorobenzenesulfonate, to form a red chromogen in the presence of an oxidizing agent, such as bilrubin oxidase.

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