Novel alkaline protease

Details Novel alkaline protease Novel alkaline protease

1983-09-28

1986-04-08

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435219, 435824, C12N 952, C12N 950, C12R 1025

Patent

active

045813329

ABSTRACT:
A novel protease is described. The enzyme has the following characteristics: molecular weight: 30,000 (a gel filtration method using Sephadex G-75); isoelectric point, 5.3; pH reactivity: the optimum pH for its esterase activity is at pH 8.5 and the optimum pH for its amidase activity is at pH 9.0 respectively; substrate reactivity: it selectively and specifically hydrolyzes the ester bond and the amide bond derived from the carboxyl group of L-lysine; inhibitor: it is inhibited by diisopropyl phosphofluoride, tosyl-L-lysine chloro-methyl ketone and phenylmethylsulfonyl chloride. The protease is preferably produced by a microorganism belonging to the genus Achromobacter.

REFERENCES:
patent: 4320197 (1982-03-01), Morihara et al.
Agricultural and Biological Chemistry 42 (7) 1443-1445 (1978).
Biochemical Biophysical Research Communications vol. 92 pp. 396-402, (1980).

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