Notched spacer for slab-gel electrophoresis

Details Notched spacer for slab-gel electrophoresis Notched spacer for slab-gel electrophoresis

1994-03-07

1995-01-24

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

C25B 900

Patent

active

053840259

ABSTRACT:
The present invention is directed to improved side spacers for use in slab gel electrophoresis that prevent the formation of channels between the electrophoresis gel and the side spacer and methods employing the side spacer. The improvement consists of forming the side spacer with one or more notches cut into the edge of the side spacer in contact with the gel, such that, when the gel hardens, the side spacer is anchored into the gel, thereby preventing the formation of channels between the side spacer and the gel.

REFERENCES:
patent: 4762743 (1988-08-01), von Alven et al.
patent: 4811218 (1986-06-01), Hunkapiller et al.
Trainor Anal. Chem., 62: 418-426, 1990 DNA Sequencing, Automation, and the Human Genome.
Watkins Biotechniques, 6: 310-319, 1988 Restriction Fragment Length Polymorphism (RFLP): Applications in Human Chromosome Mapping and Genetic Disease Research.

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