Normalizing and amplifying RNA

Details Normalizing and amplifying RNA Normalizing and amplifying RNA

2006-01-03

2006-01-03

Jones, W. Gary (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S015000, C435S091100, C435S091200, C435S091210, C435S091500, C536S024330

Reexamination Certificate

active

06982143

ABSTRACT:
The invention provides methods and compositions for normalizing and amplifying RNA populations. The methods generally comprise the steps of copying message RNA (mRNA) to form first single-stranded (ss) cDNA; converting the first ss-cDNA to first double-stranded (ds) cDNA; linearly amplifying the first ds-cDNA to form first amplified RNA (aRNA); tagging the 3′ end of the first aRNA with a known sequence to form 3′-tagged first aRNA; copying the 3′-tagged first aRNA to form second ss-cDNA; and normalizing the mRNA and/or the first aRNA.

REFERENCES:
patent: 5514545 (1996-05-01), Eberwine
patent: 5591575 (1997-01-01), Hampson et al.
patent: 5866327 (1999-02-01), Gudkov et al.
patent: 6114152 (2000-09-01), Serafini et al.
Stratagene Catalog, 1995, p. 109.

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