Normalized cDNA libraries

Details Normalized cDNA libraries Normalized cDNA libraries

1995-06-06

1997-06-10

Horlick, Kenneth R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 435 911, 536 254, C07H 2104

Patent

active

056376855

ABSTRACT:
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

REFERENCES:
patent: 5482845 (1996-01-01), Soares et al.
Ko, M.S.H., An `equalized cDNA library` by the reassociation of short doubled-stranded cDNAs, Nucleic Acids Research, 18(19):5705-5711 (1990).
Patanjali, S.R., et al., Construction of a uniform-abundance (normalized) cDNA library, Proc. Natl. Acad. U.S.A. 88:1943-1947 (1991).
NTIS Progress Report, Soares & Efstratiadis, Oct. 1992.

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