Radiant energy – Calibration or standardization methods
Patent
1992-09-10
1994-06-14
Fields, Carolyn E.
Radiant energy
Calibration or standardization methods
250328, 250361C, G01N 2176
Patent
active
053212614
ABSTRACT:
A normalization system for multi-photodetector single photon counting luminescent measuring systems and a standard for use in the same. The method comprises calculating one or more counting ratios for each photodetector in the single photon counting system and multiplying counts of unknown samples by the inverse of the appropriate counting ratio. A counting ratio for a particular photodetector is calculated by dividing the count of a standard measured by the photodetector by the count of the same standard measured by a reference photodetector. The particular standard developed employs calcium tungstate:lead as a scintillator and tritium or carbon-14 thymidine as a means of exciting the scintillator. This standard has the advantages of providing a good model of typical luminescence chemistry and producing an emission pattern which is susceptible to single photon counting.
REFERENCES:
patent: 3833827 (1974-09-01), Shaffer
patent: 4039889 (1977-08-01), Vicai
patent: 4093857 (1978-06-01), Lapidus
patent: 4588897 (1986-05-01), Inbar et al.
patent: 4703574 (1987-11-01), Garjian
Mendenhall, "The Autoradioluminescence of Uranyl Salts", Journal of Photochemistry & Photobiology,A:Chemistry, 52, (1992), pp. 285-302.
Hasting et al., "Total Quantum Flux of Isotropic Sources", Journal of Optical Society of America, (1963), vol. 53, #11, pp. 1410-1414.
Lee et al., "Quantum Yields of the Luminol Chemiluminescence Reaction in Aqueous and Aprotic Solvents", Photochemistry and Photobiology, (1972), vol. 15, pp. 227-237.
Bezman et al., "Construction and Calibration of an Apparatus for Absolute Measurement of Total Luminescence at Low Levels", (1991), Analytical Chemistry, vol. 43, No. 13, pp. 1749-1753.
Seliger, "Excited States and Absolute Calibrations in Bioluminescence", Methods in Enzymology, vol. LVII, pp. 560-601.
Seliger, "Single Photon Counting and Spectroscopy of Low Intensity Chemiluminescent Reactions", Liquid Scintillation Counting Recent Applications & Development, vol. II, (1980), Academic Press, pp. 281-319.
O'Kane et al., "Encapsulated Radiophosphorescent Standards for Day-to-Day Photometer Calibration", (1990), Photochemistry and Photobiology, vol. 52, No. 4, pp. 723-734.
Sale literature of Biolink Technology Ltd., "Luminescence Standards Light Test Standards Systems-Biolink", (no date).
Fields Carolyn E.
Packard Instrument Company Inc.
LandOfFree
Normalization technique for photon-counting luminometer does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Normalization technique for photon-counting luminometer, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Normalization technique for photon-counting luminometer will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1251108