Nonwoven fabric coated with a mixture of silk fibroin, gelatin a

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...

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435178, 435179, 435180, 43524023, 435240243, C12N 1102, C12N 500, C12N 1112, C12N 1108

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053858360

ABSTRACT:
A carrier for adhering animal cells during culturing or for immobilization of animal cells is produced by coating a porous substrate with a cell adhesive material in the form of a mixture with chitosan. In a preferred embodiment, the porous substrate is a nonwoven fabric prepared by impregnating a nonwoven fabric web with a binder resin, and the mixture contains silk fibroin, gelatin and chitosan. Coating is carried out by contacting the nonwoven fabric with a solution prepared by adding silk fibroin and gelatin to an acidic aqueous solution of chitosan to coat the nonwoven fabric, drying the coated nonwoven fabric and treating the dried nonwoven fabric with an alkali to render the chitosan insoluble.

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