Noninvasive diagnosis for allograft rejection

Details Noninvasive diagnosis for allograft rejection Noninvasive diagnosis for allograft rejection

1995-08-07

1997-06-03

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 30, C12Q 148

Patent

active

056353650

ABSTRACT:
The present invention provides a noninvasive method for diagnosing and/or predicting a rejection episode in a transplant patient, for example, in a heart transplant patient, by determining a rise in the frequency of thioguanine-resistant mutant peripheral blood mononuclear cells. Those resistant mutant cells have a deficiency in the purine salvage enzyme hypoxanthine guanine phosphoribosyltransferase. The relative frequency of the mutant cells in the population from a sample can be determined by direct plating in the presence of a selection medium and by determining of the mutation by polymerase chain reaction technology.

REFERENCES:
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