Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1992-08-07
1995-12-12
Beisner, William H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 721, 435 703, 4352402, 43524023, 435960, 435 723, 435 32, 436 63, C12Q 102, C12Q 100, C12N 500, G01N 3348
Patent
active
054749093
ABSTRACT:
A method of evaluating the effectiveness of drugs in inhibiting the growth of tumor cells. A sample of a tumor is histocultured, a drug to be evaluated is added to the sample and the sample is incubated. A suitable tetrazolium salt is added and a frozen section of the sample is prepared. The section is stained with a fluorescent dye. The section is exposed to polarized light and the reflected light is measured. Then the section is exposed to fluorescent light and the light emitted by the dye is measured. These measurements are compared to a control and/or measurements from tests using other drugs and the relative effectiveness of the drug is evaluated, preferably by pixel analysis.
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Connors Kenneth M.
Guo Hui-Yan
Anticancer Inc.
Beisner William H.
Fitting Thomas
Leary Louise N.
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