Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1981-12-11
1984-08-14
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 29, 435 34, 435240, 435235, 435948, 4351721, 935 57, C12Q 168, C12Q 129, C12N 1500, C12N 500
Patent
active
044657692
ABSTRACT:
A non-transformed cell line is produced by treating BALB/c derived 10E2 cells with 5-bromodeoxyuridine and 5-iodo-2'-deoxyuridine to produce thymidine kinaseless cells which upon multiple cloning show a flat epitheloid appearance indicative of their non-transforming potential. These cells are used to determine the tumorigenic transforming potential of any gene by introducing the gene into the cells of the non-transformed cell line along with the Herpes simplex virus thymidine kinase gene which serves as a vehicle for cotransfection. The transformation of the cells is indicative of tumorigenic potential.
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Hampar Berge
Showalter Stephen D.
Roberts, Jr. John S.
Tarcza John Edward
The United States of America as represented by the Department of
Wiseman Thomas G.
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