Non-toxic mutants of pathogenic gram-negative bacteria

Details Non-toxic mutants of pathogenic gram-negative bacteria Non-toxic mutants of pathogenic gram-negative bacteria

2007-06-05

2007-06-05

Devi, S. (Department: 1645)

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

C424S093400, C424S200100, C424S256100, C424S234100, C424S184100, C424S203100, C435S069300, C435S069100, C435S071100, C536S123100

Reexamination Certificate

active

11087235

ABSTRACT:
A method is provided for identifying, isolating, and producing htrB mutants of gram-negative bacterial pathogens. The method comprises mutating the htrB gene of a gram-negative bacterial pathogen so that there is a lack of a functional HtrB protein, resulting in a mutant that lacks one or more secondary acyl chains and displays substantially reduced toxicity as compared to the wild type strain. Also, the present invention provides methods for using a vaccine formulation containing the htrB mutant, or the endotoxin isolated therefrom, to immunize an individual against infections caused by gram-negative bacterial pathogens by administering a prophylactically effective amount of the vaccine formulation.

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