Non-toxic mutants of pathogenic gram-negative bacteria

Details Non-toxic mutants of pathogenic gram-negative bacteria Non-toxic mutants of pathogenic gram-negative bacteria

2006-02-28

2006-02-28

Devi, S. (Department: 1645)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Conjugate or complex

C424S234100, C424S256100, C424S193100, C424S831000, C424S184100, C435S091500, C536S127000, C536S123100

Reexamination Certificate

active

07005129

ABSTRACT:
A method is provided for identifying, isolating, and producing htrB mutants of gram-negative bacterial pathogens. The method comprises mutating the htrB gene of a gram-negative bacterial pathogen so that there is a lack of a functional htrB protein, resulting in a mutant that lacks one or more secondary acyl chains contained in the wild type gram-negative bacterial pathogen, and displays substantially reduced toxicity as compared to the wild type strain. Also, the present invention provides methods for using a vaccine formulation containing the htrB mutant, the endotoxin isolated therefrom, or the endotoxin isolated therefrom which is then conjugated to a carrier protein, to immunize an individual against infections caused by gram-negative bacterial pathogens by administering a prophylactically effective amount of the vaccine formulation.

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