Non-toxic mutants of pathogenic gram-negative bacteria

Details Non-toxic mutants of pathogenic gram-negative bacteria Non-toxic mutants of pathogenic gram-negative bacteria

2005-05-03

2005-05-03

Devi, S. (Department: 1645)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Bacterium or component thereof or substance produced by said...

C424S200100, C424S234100, C435S069300, C435S252300, C530S390100

Reexamination Certificate

active

06887483

ABSTRACT:
A method is provided for identifying, isolating, and producing htrB mutants of gram-negative bacterial pathogens. The method comprises mutating the htrB gene of a gram-negative bacterial pathogen so that there is a lack of a functional HtrB protein, resulting in a mutant that lacks one or more secondary acyl chains and displays substantially reduced toxicity as compared to the wild type strain. Also, the present invention provides methods for using a vaccine formulation containing the htrB mutant, or the endotoxin isolated therefrom, to immunize an individual against infections caused by gram-negative bacterial pathogens by administering a prophylactically effective amount of the vaccine formulation.

REFERENCES:
patent: 4912094 (1990-03-01), Myers et al.
patent: 4929604 (1990-05-01), Munford et al.
patent: 5200184 (1993-04-01), Munford et al.
patent: 5641492 (1997-06-01), Sprouse et al.
patent: 6482807 (2002-11-01), Van der Ley et al.
patent: 6548287 (2003-04-01), Powell et al.
patent: 9718837 (1997-05-01), None
patent: WO 9719688 (1997-05-01), None
Raetz CRH. Annu. Rev. Biochem. 59: 129-170, 1990.*
Jachymek W. Post. Hih. Med. Dosw. 49: 1: 171-178, 1995.*
Kulshin et al., J. Bacteriol. 174: 1793-1800, 1992.*
Westphal et al. Methods in Carbohydr. Chem. 5: 83-91, 1965.*
McLaughlin et al. J. Bacteriol. 174: 6455-6459, 1965.*
Brooks et al. Jawetz, Melnick & Adelberg's Medical Microbiology 20thed. 1995.*
Karow, Margaret Lee. Molecular Genetics of theEscherichia coliHtrB gene. (Dissertation, 1992).*
Karow et al Journal of Bacteriology 174(22): 7407-7418, 1992.*
Lehmann et al. Eur. J. Biochem 178:459-464, 1988.*
Lee et al. Journal of Bio.*
Hitchock et al. “Morphological Heterogeneity AmongSalmonellaLipopolysaccharide Chemotypes in Silver-Stained Polyacrylamide Gels”, Journal of Bacteriology, Apr. 1983, pp 269-277 vol. 154 No. 1.*
Golenbock, D.T., et al., “Lipid A-like molecules that antagonize the effects of endotoxins on human monocytes”,J. of Biological Chemistry, 266(29), pp. 19490-19498, (1991).
Lee, N., et al., “Mutation of the htrB Locus of Haemophilus influenzae Nontypable Strain 2019 Is Associated with Modifications of Lipid A and Phosphorylation of the Lipo-oligosaccharide”,J. Biol. Chem., 270(45). pp. 27151-27159, (1995).
Raetz, C. R., “Bacterial Endotoxins: Extraordinary lipids that activate eucaryotic signal transduction”,J. of Bacteriology, 175(18), pp. 5745-5753, (Sep. 1993).
Gupta, R.K., et al., “Synthesis, characterization, and some immunological properties of conjugates composed of the detoxifiedlipopolysaccharide of vibrio cholerae 01 serotype inaba bound to cholera toxin”,Infection and Immunity, 60, 8,3201-3208 (1992).
Karow, M., et al., “Sequencing, mutational analysis, and transcriptional regulation of theEscherichia colihtrB gene”,Molecular Microbiology, 5, 9,2285-2292 (1991).
Karow, M., et al., “Isolation and Characterization of theEscherichia colihtrB gene, whose product is essential for bacterial viability above 33degreesC in rich media”,Journal of Bacteriology,vol. 173, No. 2, pp. 741-750, (Jan. 1991).
Karow, M., et al., “The lethal Phenotype 'caused by null mutations in theescherichia colihtrB gene is suppressed by Mutations in the accBC Operon, Encoding two subunits of acetyl coenzyme A carboxylase”,Journal of Bacteriology,vol. 174, No. 22, pp. 7407-7418, (Nov. 1992).
Lee, N., et al., “Mutation of the htrB locus of Haemophilus influenzae nontypable strain 2019 is associated with modifications of lipid A and physophorylation of the lipo-oligosaccharide”,The Journal of Biological Chemistry,vol. 270, No. 45, pp. 27151-27159, (Nov. 10, 1995).
Karow et al., “The Lethal Phenotype Caused by Null Mutations in theEscherichia coli htrBGene is Suppressed by Mutations in theaccBCOperon, Encoding Two Subunits of Acetyl Coenzyme A Carboxylase” Journal of Bacteriology, vol. 174, No. 22, 11/92, pp. 7407-7418.

Affiliated with

Also associated with

Rating

Non-toxic mutants of pathogenic gram-negative bacteria does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Non-toxic mutants of pathogenic gram-negative bacteria, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Non-toxic mutants of pathogenic gram-negative bacteria will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3443035