Non toxic compositions and methods useful for the extraction of

Details Non toxic compositions and methods useful for the extraction of Non toxic compositions and methods useful for the extraction of

1993-11-23

1995-02-28

Geist, Gary

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-o

G01N 3350

Patent

active

053936728

ABSTRACT:
This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase. Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction. The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.

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