Non-sporulating Bacillus subtilis having parts of the gene...

Details Non-sporulating Bacillus subtilis having parts of the gene... Non-sporulating Bacillus subtilis having parts of the gene...

2008-01-15

2008-01-15

Woitach, Joseph (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S007320, C435S252100

Reexamination Certificate

active

07319030

ABSTRACT:
A non-sporulatingBacillus subtilisstrain, a method for preparing it and its use as a production organism. A method for preparing a biologically prepared product with the non-sporulating bacterium is also disclosed.

REFERENCES:
patent: 2004/0248279 (2004-12-01), Sawada et al.
patent: WO 9703185 (1997-01-01), None
Sambrook and Russell. Molecular Cloning: A Laboratory Manual. In vitro Amplification of DNA by the Polymerase Chain Reaction. vol. 2, Ch. 8, pp. 8.1-18.413. 2001.
Saunders et al. Use of Chromosomal Integration in the Establishment and Expression of blaZ, aStaphylococcus aureusbeta-lactamase Gene, inBacillus subtilis. J. of Bacteriology, 157(3): 718-726. 1984.
Pedraza-Reyes et al. Temporal Regulation and Forespore-Specific Expression of the Spore Photoproduct Lyase Gene by Sigma-G RNA Polymerase duringBacillus subtilisSporulation. J. Bac. 176(13): 3983-3991. 1994.
Biotechnology Letters, vol. 23, 2001, June-Hyung Kim et al., “Construction of spore mutants of Bacillus subtilis for the development as a host for foreign protein production,” pp. 999-1004.

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