Non-specific protein staining using merocyanine dyes

Details Non-specific protein staining using merocyanine dyes Non-specific protein staining using merocyanine dyes

1995-05-19

1997-04-01

Soderquist, Arlen

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436 87, 436 88, 436164, 436166, 436172, 436175, 436177, 422 61, G01N 3348, G01N 3352, G01N 3368

Patent

active

056165021

ABSTRACT:
The present invention describes the use of a variety of merocyanine dyes and substituted merocyanine dyes for detecting and quantifying poly(amino acids), including peptides, polypeptides and proteins. The labeled proteins or peptides are highly colored, but are also detected by their strong fluorescence enhancement. Poly(amino acids) are detected in solution, in electrophoretic gels, and on solid supports, including blots and dipsticks. The present method of staining is highly sensitive, extremely facile, and relatively non-selective.

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