Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1995-06-29
1998-07-21
Huff, Sheela
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
436523, 436524, 436525, 436526, 435 4, 435 6, 435 72, 435 79, 435 791, 435 792, 435 793, 435 794, 435 795, G01N 33543, G01N 33551, G01N 33553, C12G 100
Patent
active
057834539
ABSTRACT:
The assay described herein is predicated on an observation that when acridinium ester labeled tracers are bound to their corresponding binding conjugate immobilized on a metal oxide solid phase, the measurable chemiluminescent light emission of the labeled tracer bound to the solid phase is quenched as compared to the free fraction tracer that is unattached to the solid phase. According to the invention, a non-separation specific binding assay to detect or quantify the presence of an analyte is provided where the entire reaction mixture is flashed (including unreacted tracer) and modulated signal (because of the quench effect) is associated with a reference, thus determining the amount or presence of said analyte in said sample. Disadvantages inherent in heterogeneous assays employing multiple separations may be avoided using this non-separation method.
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Barlow Eve H.
Carroll, III Eddie
Connolly Joseph E.
Lee Michael J.
Martinelli Richard A.
Blackburn Robert P.
Chiron Diagnostics Corporation
Huff Sheela
Lauder Leona L.
Morgenstern Arthur S.
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