Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-08-19
1997-07-01
Hutzell, Paula K.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351721, 4352531, 435245, 4352523, 4352528, 4352524, 4352521, 435243, 435252T, 435 711, 4241841, 424 931, 4242581, 4242341, 4242401, 4242491, 4242821, C12N 1500, C12N 120, C12N 100, C12P 2104, A01N 6300, A01N 6500, A61K 3900, A61K 39108
Patent
active
056437714
ABSTRACT:
Live vaccines are provided and methods for preparing the live vaccines for protection of a host from a pathogenic microorganism. The vaccines are prepared by introducing at least one modification in a gene involved in at least one, normally at least two, biosynthetic pathways involving the production of products which are unlikely to be found in the disease susceptible host. The modification results in a gene change which cannot be repaired by a single step, e.g. polynucleotide deletions and inversions. Where the aro gene suffers such a change, the resultant auxotrophic mutants require aromatic amino acids, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid or a highly concentrated source of absorbable iron. The auxotrophic mutations have substantially reduced or nonexistent virulence while retaining the desired immunogenicity to initiate the immunogenic response. Various techniques can be employed for providing the desired change.
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Hutzell Paula K.
Minnifield N. M.
The Board of Trustees of the Leland Stanford Junior University
Trecartin Richard F.
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