Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1994-01-21
1996-12-03
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 23, 435 17, 435 15, 435 18, 435 4, 435 21, 435 16, 435810, 435975, 436 2, 436 12, 436 13, 436 14, 436 15, 436 16, 436 17, 436 63, 436 74, 436172, 436800, 530300, C12Q 137, C12Q 150, G01N 3100, A61K 3800
Patent
active
055807470
ABSTRACT:
The present invention is directed to the assay and purification of proteins, and particularly to the non-radioactive assay and purification of protein kinases, phosphatases and protease by incubating the enzyme with a substrate modified peptide to form a product modified peptide under conditions where the enzyme is active. The product modified peptide and substrate modified peptide are then separated, and the product modified peptide is measured. The present invention is also directed to kits and bioreagents for performing the assays.
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Shultz John W.
White Douglas H.
Kight John
Leary Louise N.
Promega Corporation
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