Non-radioactive DNA sequencing

Details Non-radioactive DNA sequencing Non-radioactive DNA sequencing

1995-06-07

1997-08-05

Campbell, Eggerton A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

204462, 204613, C12Q 168, G01N 2726

Patent

active

056541490

ABSTRACT:
A new process and kit are described that combines methods for generating the nucleotide base sequence of a DNA molecule with an ultra-sensitive silver staining protocol. This new combination of technologies allows for a direct, non-instrument based visualization of electrophoretically separated sequencing fragments. This non-radioactive system includes sequencing the DNA molecule by forming a set of fragments using an enzymatic dideoxy-mediated chain termination method, electrophoretically separating the DNA fragments on a gel medium, and exposing the gel medium to ultra-sensitive silver-staining solutions for a time determined by viewing the silver stain reacted primer extension products.

REFERENCES:
Beidler et al, Anal. Biochem 126(2): 374-380 (1982).
Bassom et al, Anal. Biochem 196:80-83 (1991).
Maxam et al, PNAS 74: 560-564 (1977).
Sanger et al, PNAS 74: 5463-5467 (1977).

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