Non-primate vitreal replacement model

Drug – bio-affecting and body treating compositions – In vivo diagnosis or in vivo testing – Magnetic imaging agent

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514912, 514 54, A61K 4900

Patent

active

051026538

ABSTRACT:
This is a non-primate vitreal replacement process that quantitates the inflammatory response to viscoelastic agents or solutions. More specifically, the rabbit vitreal replacement process consists of the surgical replacement of a small volume of the central gel vitreous of the rabbit with a viscoelastic agent or solution, and measuring the invasion of white blood cells in the vitreous and anterior chamber at 48 hours post injection. Additional parameters measured are anterior chamber flare, haze and flare in the vitreal chamber, ocular fundus clarity, presence of iris congestion, lens involvement, corneal clarity, conjunctival congestion and conjunctival swelling. In this process, the rabbit is evaluated prior to injection of the viscoelastic agent or solution and 48 hours post injection. Each parameter is evaluated on a scale of 0 to 5. A non-inflammatory sample consists of a mean total score of equal to or less than one or a vitreal cell count less than 50 cells/mm.sup.3. This is an extremely sensitive system for detecting small amounts of inflammatory agents in viscoelastic agents or solutions. The test solution is rated as acceptable for veterinary or human use if a non-inflammatory response is obtained in this process.

REFERENCES:
patent: 4716154 (1987-12-01), Malson et al.
patent: 4819617 (1989-04-01), Goldberg et al.
"Replacement of the Vitreous Body in the Monkey by Reconstituted Vitreous and by Hyaluronic Acid", by Balax and Sweeney, Modern Problems in Ophthalmology, vol. 4, pp. 230-232.
"The Injection of Hyaluronic Acid and Reconstituted Vitreous into the Vitreous Cavity", by Balazs and Sweeney, New and Controversial Aspects of Retinal Detachment, pp. 371-376.
"Studies on the Structure of the Vitreous Body. VIII. Comparative Biochemistry", by Balazs, Laurent, Laurent, DeRoche and Bunney, Archives of Biochemistry and Biophysics, 81, 464-479.

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