Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-04-04
2006-04-04
Ketter, James (Department: 1636)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023200, C536S023400, C435S004000, C435S006120, C435S320100, C435S325000, C435S243000, C435S440000
Reexamination Certificate
active
07022826
ABSTRACT:
A non-oligomerizing fluorescent protein, which is derived from a fluorescent protein having at least one mutation that reduces or eliminates the ability of the fluorescent protein to oligomerize, is provided. The non-oligomerizing fluorescent protein can be derived from a naturally occurring green fluorescent protein, a red fluorescent protein, or other fluorescent protein, or a fluorescent protein related thereto. Also provided is a fusion protein, which includes a non-oligomerizing fluorescent protein linked to at least one polypeptide of interest. In addition, a polynucleotide encoding a non-oligomerizing fluorescent protein is provided, as is a recombinant nucleic acid molecule, which includes polynucleotide encoding a non-oligomerizing fluorescent protein operatively linked to at least a second polynucleotide. Vectors and host cells containing such polynucleotides also are provided, as are kits containing one or more non-oligomerizing fluorescent proteins or encoding polynucleotides or constructs derived therefrom. Further provided are methods of making and using the proteins and polynucleotides.
REFERENCES:
patent: 5625048 (1997-04-01), Tsien et al.
patent: 2003/0022287 (2003-01-01), Lukyanov et al.
patent: WO 96/23810 (1996-08-01), None
patent: WO 00/34326 (2000-06-01), None
patent: WO 00/34526 (2000-06-01), None
patent: WO 01/62919 (2001-08-01), None
patent: WO 01/96373 (2001-12-01), None
Crameri et al. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotechnology 14:315-319, Mar. 1996.
Ormö, Mats, et al., “Crystal Structure of theAequorea victoriaGreen Fluorescent Protein”, Science, vol. 273, pp 1392-1395, Sep. 6, 1996.
Yanushevich, Yurii G., et al., “A Strategy for the Generation of Non-Aggregating Mutants ofAnthozoaFluorescent Proteins”, FEBS Letters 511, pp 11-14, 2002.
Campbell et al. “A monomeric red fluorescent protein”PNAS99(12):7877-7882 (2002).
Tsien, R. “Rosy dawn for fluorescent proteins”Nature Biotechnology17: 956-957 (1999) and Nucleotide search results, Accession No. AF168419.
Yang et al., The Molecular Structure of Green Fluorescent Protein.Nature Biotechnology14(10): 1246-51 (1996).
Baird et al., “Circular Permutation and Receptor Insertion within Green Fluorescent Proteins”Proc. Natl. Acad. Sci USA96:11241-11246 (1999).
BD Biosciences/Clontech, Product literature, “Living Colors DsRed C-Terminal Fusion Vector”CLONTECHniques, p. 22, (Jan. 2000).
BD Biosciences/Clontech, Product literature, “Living Colors Red Fluorescent Protein”CLONTECHniques, vol. XIV, No. 4 pp. 2-6, (Oct. 1999).
BD Biosciences/Clontech, Product literature, “Mercury DsRed1 Signaling Probes”CLONTECHniques, pp. 22 & 23, (Apr. 2000).
Heim et al., “Improved Green Fluorescence”Nature, 373:663-664, 1995.
Heim et al., “Wavelength Mutations and Posttranslational Autoxidation of Green Fluorescent Protein”,Proc. Natl. Acad. Sci. USA, 91:12501-12504, 1994.
Lukyanov et al., “Natural Animal Coloration Can Be Determined by a Nonfluorescent Green Fluorescent Protein Homolog”J. Biol. Chem., 275:25879-25882, 2000.
Miyawaki and Tsien, “Monitoring Protein Conformations and Interactions by Fluorescene Resonance Energy Transfer Between Mutants of Green Fluorescent Protein”Methods Enzymol., Application of Chimeras in Cell Physiology, 327:472-500 (2000).
Miyawaki et al., “Dynamic and Quantitative Ca2+ Measurements Using Improved Cameleons”Proc. Natl. Acad. Sci., USA, 96:2135-2140, 1999.
Prasher et al., “Summary Structure of the Aequorea Victoria Green-Fluorescent Protein”Gene, 111:229-233 (1992).
Roth, “Purification & Protease Susceptibility of the Green-Fluorescent Protein of Aequorea with a Note on Hlistaura” Thesis from the Graduate Program in Biochemistry from Rutgers, the State University on New Jersey (1985).
Wall et al., “The Structural Basis for Red Fluorescence in the Tetrameric GFP Homolog DsRed”Nature Struct. Biol., 7:1133-1138 [2000].
Wiehler et al., “Mutants of Discosoma Red Fluorescent Protein with a GFP-like Chromophore”FEBS Letters487: 384-389 (2001).
Yarbrough et al., “Refined Crystal Structure of DsRed, a Red Fluorescent Protein from Coral, at 2.0—A Resolution”Proc. Natl. Acad. Sci. USA, 98:462-467 [2000].
Baird, et al., “Biochemistry, Mutagenesis, and Oligomerization of DsRed, a Red Fluorescent Protein from Coral,”Proceedings of the National Academy of Sciences of the United States of America, 97(22):11984-11989 (Oct. 24, 2000).
Gross et al., “The Structure of the Chromophore within DsRed, a Red Fluorescent Protein from Coral,”Proceedings of the National Academy of Sciences of the United States of America, 97(22):11990-11995 (Oct. 24, 2000).
Matz, et al., “Fluorescent Proteins from Nonbioluminescent Anthozoa Species,”Nature Biotechnology, 17:969-973 (1999).
Baird Geoffrey S.
Tsien Roger Y.
Zacharias David A.
Dreger Ginger R.
Fox James A.
Heller Ehrman LLP
Ketter James
Lamberston David A.
LandOfFree
Non-oligomerizing fluorescent proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Non-oligomerizing fluorescent proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Non-oligomerizing fluorescent proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3550852