Non-nucleoside 1,3-diol reagents for labeling synthetic oligonuc

Details Non-nucleoside 1,3-diol reagents for labeling synthetic oligonuc Non-nucleoside 1,3-diol reagents for labeling synthetic oligonuc

1993-06-03

1995-09-19

Robinson, Douglas W.

Stock material or miscellaneous articles

Coated or structually defined flake, particle, cell, strand,...

Particulate matter

558192, 560155, 568933, C07C22900, C07D21900, C07D31188, C07F 922

Patent

active

054514630

ABSTRACT:
A trifunctional reagent possessing a primary hydroxyl, a secondary hydroxyl, and a primary amino group. This reagent is useful in solid phase oligonucleotide synthesis for the convenient labeling of the 3'-terminus. The secondary hydroxyl may be an phosphoramidite derivative permitting the attachment to the solid phase support. The reporter molecule may be attached to the trifunctional molecule prior to the completion of the oligonucleotide synthesis or after the oligonucleotide is cleaved from the support. A preferred species of this trifunctional molecule is shown below.

REFERENCES:
Nelson et al. Nucl. Acids Res. 17:7179-7186, 1989.
Misiura et al. Nucl. Acids Res. 18:4345-4354, 1990.
Zuckerman, et al. Nucl. Acids Res. 15:5305-5321, 1987.
Smith, L. M., S. Fung, M. W. Hunkapillar, T. K. Hunkapillar, L. E. Hood (1985) "The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis," Nucl. Acids Res. 13(7):2399-2412.
Cocuzza, A. J. (1989) "A phosphoramidite reagent for automated solid phase synthesis of 5'-biotinylated oligonucleotides," Tetrahedron Letters 30(46):6287-6290.
Hultman, T., S. Bergh, T. Moks, M. Uhlen (1991) "Bidirectional Solid-Phase Sequencing of In Vitro-Amplified Plasmid DNA," BioTechniques 10(1):84-93.
Landgraf, A., B. Reckmann, A. Pingoud (1991) "Quantitative Analysis of Polymerase Chain Reaction (PCR) Products Using Primers Laabeled with Biotin and a Fluorescent Dye," Analytical Biochemistry 193:231-235.
Connell, C., S. Fung, C. Heiner, J. Bridgham, V. Chakerian, E. Heron, B. Jones, S. Menchen, W. Mordan, M. Raff, M. Recknor, L. Smith, J. Springer, S. Woo, M. Hunkapillar (1987) "Automated DNA Sequence Analysis," BioTechniques 5(4):342-346.
Zimran, A., C. Glass, V. S. Thorpe, E. Beutler (1989) "Analysis of`color PCR` by automatic DNA sequencer," Nucl. Acids Res. 17(18):7538.
Kaiser, R. J., S. L. MacKellar, R. S. Vinayak, J. Z. Sanders, R. A. Saavedra, L. E. Hood (1989) "Specific primer-directed DNA sequencing using automated fluorescence detection," Nucl. Acids Res. 17(15):6087-6102.

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