Non-melanotytic mammalian cell constitutively expressing biologi

Details Non-melanotytic mammalian cell constitutively expressing biologi Non-melanotytic mammalian cell constitutively expressing biologi

1995-01-23

1998-06-30

Hendricks, Keith D.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435352, 435357, 435363, 435366, 435128, 435133, 435189, C12N 510, C12N 516, C12N 518, C12N 522

Patent

active

057732914

ABSTRACT:
This invention provides a non-melanocytic eucaryotic cell constitutively expressing biologically active human tyrosinase. The present invention also provides methods of producing biologically active human tyrosinase. Additionally, the invention provides a non-melanocytic eucaryotic cell which constitutively expresses biologically active human tyrosinase which in turn catalyzes the production of melanin. The melanin so produced may then be recovered.

REFERENCES:
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patent: 5529909 (1996-06-01), della-Cioppa et al.
Bouchard, B. et al. Journal of Experimental Medicine 169: 2029-2042 (Exhibit B).
Muller, G. et al. EMBO 7 (9): 2723-2730 (Exhibit C).
Shibahara, S. et al. Nucleic Acid Research 14 (6): 2413-2417 (Exhibit D).
Takeda, A. et al. Biochemical and Biophysical Research Communications 162 (3): 984-990 (Exhibit E); and.
Wigler, M. et al. Cell 16: 777-785 a(Exhibit F).
Kwon et al, Isolation and sequence of cDNA Clone for human tyrosinase that maps at the mouse calbino locus, PNAS, v. 84, 7473-77, 1987.
Winnacker, From Genes to Clones, Introduction to Gene Technology, "Gene expression in Eukanyotes" pp. 193-204, 1988.

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