Non-linear amplitude dielectrophoresis waveform for cell fusion

Details Non-linear amplitude dielectrophoresis waveform for cell fusion Non-linear amplitude dielectrophoresis waveform for cell fusion

2005-07-12

2005-07-12

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Fusion of cells

C435S461000, C435S470000, C435S471000

Reexamination Certificate

active

06916656

ABSTRACT:
An object of the invention is to provide a method of treating biological cells prior to subjecting the biological cells to cell fusion pulses which includes the step of treating the biological cells with pre-fusion electric field waveforms which change amplitude in a non-linear way with respect to time, such that the biological cells are first aligned with a relatively low amplitude, long duration pre-fusion electric field waveform and then compressed with a relatively high amplitude, short duration pre-fusion electric field waveform resulting in increased cell membrane contact prior to being subjected to cell fusion. The non-linear pre-fusion electric field waveforms can change in a stepped way, in a continuous way, in a sigmoidal way, with step-wise increasing waveforms in adjacent steps, with step-wise increasing waveforms in non-adjacent steps, and in accordance with non-linear algorithms.

REFERENCES:
patent: 6197176 (2001-03-01), Pethig et al.

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