Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-10-03
1999-11-16
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 436501, 536 253, C12Q 168
Patent
active
059855495
ABSTRACT:
Improved methodologies for in-situ hybridization and non-isotopic detection of nucleic acid sequences are provided which offer major increases of resolution, sensitivity, and simplicity unavailable in previously known techniques. The methodology is able to detect even a single-copy of a specific nucleic acid of interest under controlled conditions regardless of whether these are DNA or RNA sequences; or whether the nucleic acid sequence of interest is localized in the chromosomes, nucleus, or cytoplasm of a cell. The methods employ a variety of non-isotopic labels and detection means for rapid and reliable assays. The invention is also provided in kit form for use in the clinical/diagnostic laboratory such that a relatively unskilled person can accurately and reproducibly detect even a single-copy of a specific nucleic acid of interest.
REFERENCES:
patent: 4419446 (1983-12-01), Howley et al.
patent: 4652517 (1987-03-01), Scholl et al.
patent: 4711955 (1987-12-01), Ward et al.
patent: 4770992 (1988-09-01), Van den Engh
patent: 4886741 (1989-12-01), Schwartz
patent: 4888278 (1989-12-01), Singer et al.
patent: 5028525 (1991-07-01), Gray et al.
patent: 5447841 (1995-09-01), Gray et al.
patent: 5597692 (1997-01-01), Coghlan et al.
Lawrence, J.B. et al., J. Cell Biol 105:Abstract No. 830 (Oct. 1987).
Bauman, J.G.J. and P.Bentvelzen, Cytometry 9:517-524 (1988).
Manuelidis (1984) Proc. Natl Acad Sci (USA) vol. 81, pp. 3123-3127.
Molecular Cloning, A Laboratory Manual (publ. Cold Spring Harbor Laboratory, New York, USA; Maniatis et al., (1982), pp. 188-189.
Landegent et al. (1984) Experimental Cell Res., vol. 153, pp. 61-72.
Rappold et al. (1984) Human Genet., vol. 67, pp. 317-325.
Lichter, P. et al., "Delineation of Individual Human Chromosomes in Metaphase and Interphase Cells by In Situ Suppression Hybridization . . . " Hum. Genet., 51 (1988).
Pinkel, D. et al., "Detection of Structural Chromosome abberations in Metaphase . . . " American Journal of Human Genetica, 43, Supl. No. 3, Abstracts (1988).
Gray, J.V. et al., "Fluorescence Hybridization to Human Chromosome 21 Using Probes From a Charon 21 A Library" Cytometry, Supplement 1, Abstracts (1987).
Langegent, J.E. et al., "Use of Whole Cosmid Clones Genomic Sequences for Chromosomal Localization by Non-Radioactive In Situ Hybridization" Hum. Genet, 77:366-370 (1987).
Sealey, Paul G. et al., "Removal of Repeated Sequences From Hybridisation Probes" Nucleic Acids Research, 13:1905-1922 (1985).
Harper, Mary E. et al., "Localization of Single Copy DNA Sequences on G-Banded Human Chromosomes by in situ Hybridization" Chromosoma, (Berl.) 83:431-439 (1981).
Lawrence, J.B. et al., "Sensitive, High-Resolution Chromatin and Chromosome Mapping In Situ: Presence and Orientation of Two Closely Integrated Copies of EBV in a Lymphoma Line" Cell, 52:51-61 (1988).
Singer, R.H. et al., "Optimization of in situ Hybridization Using Isotopic and Non-Isotopic Detection Methods" BioTechniques, 4(3):230-250 (1986).
Pinkel, D. et al., "Cytogenic Analysis Using Quantitative, High-Sensitivity, Fluorescence Hybridization" Proc. Natl. Acad. Sci. USA, 83:2934-2938 (1986).
Langer-Safer, P.R. et al. "Immunological Method for Mapping Genes on Drosophila Polytene Chromosomes" Proc. Natl. Acad. Sci. USA, 79:4381-4385 (1982).
Manuelidis, L. et al., "Chromosomal and Nuclear Distribution of the HindIII 1.9-kb Human DNA Repeat Segment" Chromosoma, (Berl.) 91:28-38 (1984).
Manuelidis, L., "In Situ Detection of DNA Sequences Using Biotinylated Probes" Focus 7:4 4-8.
Narayanswami, S. et al., "Electron Microscopic In Situ Hybridization Using Biotinylated Probes" Focus 8:1 3-6.
Brigati, D.J. et al., "Detection of Viral Genomes In Cultured Cells and Paraffin-Embedded Tissue Sections Using Biotin-Labeled Hybridization Probes" Virology, 126:32-50 (1983).
Lawrence et al., J. Cell. Biology, 99 (Oct. 1984) Abstract #519.
Singer, et al., Proc. Natl. Acad. Sci. USA, 79:7331-7335 (1982).
Gee et al., DNA, 2:157-163 (1983).
Capco et al., Developmental Biology, 67:137-151 (1978).
Angerer et al., Nucleic Acids Research, 9(12):2819-2839 (1981).
Leary, J.J. et al., Proc. Natl. Acad. Sci. U.S.A., 80:4045-4049 (Jul. 1983).
Kuo, M.T. et al., Chem. Abstract, 88(1):3872p (1978).
Brandsma, J. and G. Miller, Proc. Natl. Acad. Sci. U.S.A., 11:6851-6855 (Nov. 1980).
Godard, C.M. and K.W. Jones, Histochem., 65:291-300 (1980).
Lawrence, J.B. and R.H. Singer, Nucleic Acids Research, 13:1777-1799 (1985).
Trask, B. et al., Science, vol. 230 1401-1403 (Dec. 20, 1985).
Lawrence Jeanne B.
Singer Robert H.
Marschel Ardin H.
University of Massachusetts
LandOfFree
Non-isotopic in-situ hybridization method for detection of nucle does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Non-isotopic in-situ hybridization method for detection of nucle, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Non-isotopic in-situ hybridization method for detection of nucle will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1322836