Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Patent
1991-06-25
1993-09-28
Kepplinger, Esther L.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
436518, 436817, 436826, 435 792, 435 793, 435 794, 435 795, 435962, G01N 33543
Patent
active
052486206
ABSTRACT:
The invention concerns a method for the determination of a partner in an immunological reaction based on an immunoassay in which one of the reaction partners is present in a solid phase, wherein a polyethylene oxidepolypropylene oxide block copolymer having the formula I ##STR1## is used as the surface-active agent in which a can have a value between 40 and 150 and b a value between 10 and 50, which is characterized in that a hydrophilic block copolymer composition having the formula I is used which in a 1 % aqueous solution (weight/volume) has a surface tension of at least 45 mN/m and in which the average molar ratio of ethylene oxide groups to propylene oxide groups (2a/b) in the composition is at least 5.8. In addition the invention comprises a reagent for carrying out the above method.
REFERENCES:
patent: 4921808 (1990-05-01), Schneyer et al.
Chang et al. Anal. Biochem. 104 102-117 (1980).
Griesser Hans-Werner
Klein Christian
Kobold Uwe
Sluka Peter
Boehringer Mannheim GmbH
Green Lora Marie
Kepplinger Esther L.
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