Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai
Patent
1995-03-15
1997-12-30
Low, Christopher S.F.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Carbohydrate doai
4351723, 4351721, 4353201, 435194, 436504, 600 9, 600 1, 600 2, 600 3, 600 4, 536 232, 536 235, 536 2374, 536 231, A01N 4304, A61K 3170
Patent
active
057030561
ABSTRACT:
The subject invention provides a method of detecting gene transfer to and expression in a target tissue of a host subject comprising: (a) administering to the host subject a transfer vector containing a marker gene not naturally present in the host and nontoxic to the host, wherein the transfer vector transfects cells of the target tissue, under conditions such that the marker gene is expressed in transfected cells of the target tissue, thereby generating a marker gene product; (b) administering to the host subject a labelled marker substrate which is not metabolized by non-transfected cells, under conditions such that the marker substrate is metabolized by the marker gene product of step (a) to produce a labelled marker metabolite which is substantially retained in the transfected cells throughout a time-period sufficient for imaging the labelled marker metabolite; and (c) imaging the labelled marker metabolite, thereby detecting gene transfer to and expression in the target tissue. The subject invention provides a non-invasive, clinically applicable method for imaging gene transfer and expression which can be implemented using existing imaging techniques to monitor and evaluate in vivo gene therapy in human subjects.
REFERENCES:
Emiliana Borrelli, Richard Heyman, Mary Hsi and Ronald M. Evans, "Targeting of an inducible toxic phenotype in animal cells," Proc. Natl. Acad. Sci. U.S.A., 85, 7572-7576 (1988); U.S.A.
Kenneth W. Culver and R. Michael Blaese, "Gene therapy for cancer, " Trends in Genetics, 10, 174-178 (1994); U.S.A.
Kenneth W. Culver, Zvi Ram, Stuart Wallbridge, Hiroyuki Ishii, Edward H. Oldfield and R. Michael Blaese, "In Vivo Gene Transfer with Retroviral Vector-Producer Cells for Treatment of Experimental Brain Tumors," Science, 256, 1550-1552 (1992); U.S.A.
Marston Manthorpe, Racine Cornefert-Jensen, Jukka Hartikka, Jiin Felgner, Ann Rundell, Michal Margalith and Varavani Dwarki,"Gene Therapy by Intramuscular Injection of Plasmid DNA: Studies on Firefly Luciferase Gene Expression in Mice," Human Gene Therapy, 4, 419-431 (1993); U.S.A.
Yutaka Saito, Richard W. Price, David A. Rottenberg, Jack J. Fox, Tsann-Long Su, Kyoichi A. Watanabe and Frederick S. Philips, "Quantitative Autoradiographic Mapping of Herpes Simplex Virus Encephalitis with a Radiolabled Antiviral Drug," Science, 217, 1151-1153 (1982); U.S.A.
Frederick L. Moolten, "Tumor Chemosensitivity Conferred by Inserted Herpes Thymidine Kinase Genes: Paradigm for a Prospective Cancer Control Strategy, " Cancer Research 46, 5276-5281 (1986); U.S.A.
Frederick L. Moolten and John M. Wells, "Curability of Tumors Bearing Herpes Thymidine Kinase Genes Transferred by Retroviral Vectors," Journal of the National Cancer Institute, 82, 297-300 (1990); U.S.A.
Frederick S. Phillips, Aaron Feinberg, Ting-Chao Chou, Pedro M. Vidal, Tsann-Long Su, Kyoichi A. Watanabe and Jack J. Fox, "Distribution, Metabolism, and Excretion of 1-(2-Fluoro-2deoxy-B-D-arabinofuranosyl) thymine and 1-(2-Fluoro-2-deoxy-B-D-arabinofuranosyl) -5-iodocytosine," Cancer Research 43, 3619-3627 (1983); U.S.A.
Kendall O. Smith, Karen S. Galloway, Wiebke L. Kennell, Kevin K. Ogilvie and Bruno K. Radatus, "A New Nucleoside Analog, 9-(2-Hydroxymethyl) Ethoxy Methyl Guanine, Highly Active In Vitro Against Herpes Simplex Virus Types 1 and 2," Antimicrobial Agents and Chemotherapy, 22, 55-61 (1982); U.S.A.
Juri G. Tjuvajev, Homer A. Macapinlac, Farhad Daghighian, Andrew M. Scott, James Z. Ginos, Ronald D. Finn, Paresh Kothari, Revathi Desai, Jiaju Zhang, Bradley Beattie, Martin Graham, Stevin M. Larson and Ronald G. Blasberg, "Imaging of Brain Tumor Proliferative Activity with Iodine-131-Iododeoxyuridine," Journal of Nuclear Medicine, 35, 1407-1417 (1994); U.S.A.
Kyoichi A. Watanabe, Uri Reichman, Kosaku Hirota, Carlos Lopez and Jack J. Fox, "Nucleosides, 110. Synthesis and Antiherpes Virus Activity of Some 2'-Fluoro-2'-deoxy-arabinofuranosylpyrimidine Nucleoides," Journal of Medicinal Chemistry, 22, 21-24 (1979); U.S.A.
Singleton et al. (eds.) 1987 in: Dictionary of Microbiology and Molecular Biology, Second Edition, John Wiley & & Sons, Chichester,GB, pp. 901-904.
Froguel et al. 1995 Tibtech, 52-55.
Biological Abstracts, 90(11): AB-1214, abstract number 129728, Martelli et al. 1990, Pharmacol. Toxicol. 66(5): 329-334.
Biological Abstracts, 91(12): AB-175, abstract number 127575, Loft et al., 1991, Biochem. Pharmacol. 41(8): 1127-1134.
Biological Abstracts, 81: AB-685, abstract number 103868, Heimbrook et al. 1986, Mol. Pharmacol. 29(2): 168-172
Blasberg Ronald G.
Tjuvajev Juri
Low Christopher S.F.
Sloan-Kettering Institute for Cancer Research
White John P.
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