Non-instrumented assay with quantitative and qualitative results

Chemistry: analytical and immunological testing – Involving diffusion or migration of antigen or antibody

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422 56, 422 57, 422 58, 422 59, 422 61, 435 71, 435 72, 435 793, 435 794, 4352877, 4352886, 435969, 435970, 435973, 436518, 436530, G01N 33533

Patent

active

059982217

ABSTRACT:
The invention is directed to a non-instrumented assay giving quantitative and/or qualitative results. Specifically, the invention is directed to an assaying process wherein a first portion of binder supported on substantially the entire length of a solid is contacted with a sample suspected of containing analyte. The binder is a binder for at least the analyte. The first portion is also contacted with a tracer. The process includes contacting a second binder portion also supported on the solid with tracer. The second portion is juxtaposed to the first portion and has a known amount of analyte thereon. The amount of analyte in the sample is determined by comparing the visibility of tracer in the first and second portions. In a preferred embodiment, a third binder portion supported on the solid juxtaposed to the second portion is contacted with tracer. The third portion has a known amount of analyte thereon. The amount of analyte in the third portion is different from the amount on the second portion. According to another aspect, the invention is also directed to a kit for performing the above-described process.

REFERENCES:
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patent: 4703017 (1987-10-01), Campbell et al.
patent: 4999285 (1991-03-01), Stiso
patent: 5081013 (1992-01-01), Rovelli et al.
patent: 5158869 (1992-10-01), Pouletty et al.
patent: 5240844 (1993-08-01), Wie et al.
patent: 5308775 (1994-05-01), Donovan et al.
patent: 5356785 (1994-10-01), McMahon et al.
patent: 5424220 (1995-06-01), Goerlack-Graw et al.

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