Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Inactivation or attenuation; producing viral subunits
Patent
1994-11-14
1997-12-16
Smith, Lynette F.
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Inactivation or attenuation; producing viral subunits
435238, 435239, 435270, 424208, C12N 704, C12N 706, C12N 702, A61K 3921
Patent
active
056984308
ABSTRACT:
A method of producing a vaccine comprising providing a quantity of the disease-causing entity, treating the quantity to remove or disrupt a majority of the nucleic acid present in the quantity sufficient to render the quantity non-infective while maintaining the structure of the proteins and glycoproteins on the surface of the entity to render the vaccine protective. A method of producing a vaccine to human pathogenic retroviruses comprising the steps of partially solubilizing a quantity of the virus to produce a suspension, adding a proteinase to the suspension to release viral nucleic acids from the viral coat proteins, treating the suspension to disrupt or remove sufficient viral nucleic acids to render the suspension substantially or completely non-infective and verifying the sufficient disruption or removal of viral nucleic acids from the suspension.
REFERENCES:
patent: 5106619 (1992-04-01), Wiesehahn
patent: 5168053 (1992-12-01), Altman et al.
patent: 5242820 (1993-09-01), Lo
Charoenvit, et al. 1991, "Inability of malaria vaccine to induce . . . ," Science 251: 668-671.
Hoffman, et al, 1987, "Naturally acquired anhbodies to sporozoiles . . . " Science 231: 639-642.
Skelly, et al, 1981, "Formaldehyde treatment of hepatitis . . . " J. Virol. Methods 3: 51-59.
Fox, 1994, "No Winners against AIDS". Biotechnology 12: 128.
Rubinstein Alan I.
Rubinstein Daniel B.
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