Non-infectious HIV particles lacking long terminal repeats

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4241841, 4241851, 4241861, 4241871, 4241881, 4242041, 4242071, 4242081, 435 691, 4351723, 435 701, 435 711, 435236, 4352351, 435974, 530350, 530826, 536 231, 536 2372, 514 2, A61K 3921, C12N 1549, C07K 14155

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054398096

ABSTRACT:
An immunogenic HIV retrovirus-like particle which is non-infectious and non-replicating and which is useful as a candidate vaccine component against HIV infection, is produced by genetic engineering. A DNA molecule comprising the HIV genome devoid of long terminal repeats is incorporated into an expression vector, which is introduced into mammalian cells for expression of the HIV retrovirus-like particle.

REFERENCES:
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Gheysen, D. et al. 1989. Cell 59:103-112.
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Desrosiers, R. C. et al, 1989. Proc. Nalt. Acad. Sci. 86:6353-6357.
Bowie, J. V. et al. 1990, Science 247:1306-1310.
Lu, Y. et al. J. of Virology 63(9):4115-4119.
Sambrook, J. et al. Molecular Cloning, Cold Spring Harbor Lab Press (1989) pp. 16.7-16.72.

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