Non-immunochemical binding of lipopolysaccharides and sandwich a

Details Non-immunochemical binding of lipopolysaccharides and sandwich a Non-immunochemical binding of lipopolysaccharides and sandwich a

1987-01-21

1990-03-06

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

436502, 436518, 436827, 436511, 536127, 435264, G01N 33579, G01N 33569

Patent

active

049065670

ABSTRACT:
Sandwich assays for detecting and identifying lipopolysaccharides of gram negative bacteria utilizing immobilized lipopolysaccharide binding proteins and labelled detection reagents are provided. The active supports are also useful in removing LPS from biomedical and cosmetic preparations.

REFERENCES:
patent: 3915805 (1975-10-01), Levin
patent: 4134792 (1979-01-01), Boguslaski et al.
patent: 4683196 (1987-07-01), McLaughlin
"Endotoxin Contamination of Enzyme Conjugates Used in Enzyme-Linked Immunosorbent Assays", R. E. Bryant et al., Journal of Clinical Microbiology 17(6); 1050-1053;1983.
"Immobilized Enzymes", O. Zaborsky, p. 75,1973, CRC Press.
"Removal of Gram-Negative Endotoxin from Solutions by Affinity Chromatography", A. C. Issekutz, Journal of Immunological Methods 61;275-281; 1983.

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