Non-human primate monoclonal antibodies and methods

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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435 5, 435 7, 435 68, 4351722, 4351723, 435188, 43524027, 435948, 935 96, 424 11, 424 86, C07K 1500, C12N 500, A61K 3942, A61K 3944

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047772450

ABSTRACT:
A stable trioma cell line capable of secreting a non-human primate monoclonal antibody specific against a selected antigen. An exemplary cell line secretes chimpanzee monoclonal antibody specific against an antigen associated with hepatitis nonA
onB infection. The cell line is produced, in the method of the invention, by isolating lymphocytes from a primate immunized with the selected antigen, and immortalizing the lymphocytes by fusion with a stable, non-antibody-secreting murine myeloma/human hybridoma cell line having selected-for human characteristics. The trioma fusion products are selected for secretion of the desired antibody, which has a variety of diagnostic and/or therapeutic uses.

REFERENCES:
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patent: 4529694 (1985-07-01), Lazarus
Stanley, H. A. et al., Proc. Natl. Acad. Sci., USA, 82: 6272-6275 (9-1985).
Van Meel, F. C. M. et al., J. Immunol. Methods, 80(2): 267-276 (2-1985).
Foung, A. K. H. et al., J. Immunol. Methods 70: 83-90 (5-1984).
Wands, J. R. et al., Proc. Natl. Acad. Sci., USA, 79: 7552-7556 (12-1982).

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