Non-human carbonyl hydrolase mutants, vectors encoding same and

Details Non-human carbonyl hydrolase mutants, vectors encoding same and Non-human carbonyl hydrolase mutants, vectors encoding same and

1991-12-11

1993-01-26

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435221, 4352525, 4353201, 435832, 435 91, 4351723, 4351721, 536 232, 930240, 935 10, 935 14, 935 74, C12N 956, C12N 954, C12N 121, C12N 1555

Patent

active

051822040

ABSTRACT:
Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to encode the substitution of an amino acid in the amino acid sequence of a precursor carbonyl hydrolase. Such mutants have properties which are different than the precursor hydrolase.

REFERENCES:
patent: 4760025 (1988-07-01), Estell et al.
Rastetter; W. H. 1983 Trends Biotechnol-1, 80-84.
Wells et al. 1987, Proc. Nat'l. Acad. Sci. USA, 84, 1219-1223.
Bryan et al. 1986 Proc. Nat'l. Acad. Sci. 83, 3743-3745.
Estell et al. 1986 Science 233, 659-663.

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