Non-hemolytic mosquitocidal microorganisms

Details Non-hemolytic mosquitocidal microorganisms Non-hemolytic mosquitocidal microorganisms

1991-09-13

1994-04-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 435 712, 4351721, 4351723, 4352523, 4352525, 4353201, 435832, 536 2371, 935 10, 935 29, 935 56, 935 74, 514 2, C12N 1532, C12N 1575, C12N 121

Patent

active

053044843

ABSTRACT:
The cyta gene encoding the 28 kDa polypeptide of Bacillus thuringiensis israelensis crystals was disrupted in the 72 MDa resident plasmid by in vivo recombination. The absence of the 28 kDa protein in B. thuringiensis israelensis did not affect the crystallization of the other toxic components of the parasporal body. However, the absence of the 28 kDa protein did abolish the hemolytic activity of B. thuringiensis serovar israelensis crystals. The mosquitocidal activity of the 28 kDa protein-free crystals did not differ significantly from the wild-type crystals.

REFERENCES:
James D. Watson "Molecular Biology of the Gene" The Benjamin/Cummings Publishing Co. Menlo Park, CA. p. 313 (1987).
Bourgouin, C. et al. "Characterization of the Genes Encoding the Hemolytictoxin . . ." Mol. Gen. Genet. 205:390-397 (1986).
Delecluse, A. et al. "Deletion by In vivo Recombination . . . " J. of Bacteriology 173:3374-3381 (Jun. 1991).
A. M. Albertini et al., "Amplification Of A Chromosomal Region In Bacillus Subtilis", J. Bacteriol., 162:1203-1211 (1985).
J. L. Armstrong et al., "Delta Endotoxin Of Bacillus Thuringiensis Subsp. Israelensis", J. Bacteriol., 161:39-46 (1985).
C. Bourgouin et al., "A Bacillus Thuringiesis Subsp. Israelensis Gene Encoding A 125-Kilodalton Larvicidal Polypeptide Is Associated With Inverted Repeat Sequences", J. Bacteriol., 170:3575-3583 (1988).
P. Y. K. Cheung et al., "Separation Of Three Biologically Distinct Activities From The Parasporal Crystal Of Bacillus Thuringiensis Var. Israelensis", Curr. Microbiol., 12:121-126 (1985).
C. N. Chilcott et al., "Comparative Toxicity Of Bacillus Thuringiensis Var. Israelensis Crystal Proteins In Vivo And In Vitro", J. Gen. Microbiol., 134:2551-2558 (1985).
E. W. Davidson et al., "Isolation And Assay Of The Toxic Component From The Crystals Of Bacillus Thuringiensis Var. Israelensis", Curr. Microbiol., 11:171-174 (1984).
A. Delecluse, "Caracterisation Des Genes Codant Por Les Toxines De Bacillu Thuringiensis Serovar Israelensis Actif Sur Les Larves De Dipteres, Vecteurs De Maladies Tropicales" These de Doctorate De L'Universite Paris VII (Jan. 15, 1991).
A. Delecluse et al., "Specificity Of Action On Mosquito Larvae Of Bacillus Thuringiensis Israelensis Toxins Encoded By Two Different Genes", Mol. Gen Genet, 214:42-47 (1988).
B. A. Federici et al., "Parasporal Body Of Bacillus Thuringiensis Israelensis. Structure, Protein Composition, And Toxicity", pp. 16-44. In H. de Barjac, and D. J. Sutherland (ed.), Bacterial Control of Mosquitoes and Black Flies; Biochemistry, Genetics and Applications of Bacillus thuringiensis israelensis and Bacillus sphaericus. Rutgers University Press, New Brunswick (1990).
L. H. Goldberg et al., "A Bacterial Spore Demonstrating Rapid Larvicidal Activity Against Anopheles Sergentii, Uranotaenia Unguiculata, Culex Univitatus, Aedes Aegypti And Culex Pipiens", Mosq. News, 37:355-358 (1977).
J. M. Gonzalez, Jr. et al., "A Large Transmissible Plasmid Is Required For Crystal Toxin Production In Bacillus Thuringiensis Variety Israelensis", Plasmid, 11:28-38 (1984).
G. A. Held et al., "Effect Of Removal Of The Cytolytic Factor Of Bacillus Thuringiensis Subsp. Israelensis On Mosquito Toxicity", Biochem. Biophys. Res. Commun., 141:937-941 (1986).
H. Hofte et al., "Insecticidal Crystal Proteins Of Bacillus Thuringiensis", Microbiol. Rev., 53:242-255 (1989).
J. M. Hurley et al., "Separation Of The Cytolytic And Mosquitocidal Proteins Of Bacillus Thuringiensis Subsp. Israelensis", Biochem. Biophys. Res. Commun., 126:961-965 (1985).
J. E. Ibarra et al., "Isolation Of A Relatively Nontoxic 65-Kilodalton Protein Inclusion From The Parasporal Body Of Bacillus Thuringiensis Subs. Israelensis", J. Bacteriol., 165:527-533 (1986).
J. P. Insell et al., "Composition And Toxicity Of The Inclusion Of Bacillus Thuringiensis Subsp. Israelensis", Appl. Environ. Microbiol., 50:56-62 (1985).
L. Janniere et al., "Stable Gene Amplification In The Chromosome Of Bacillus Subtilis", Gene, 40:47-55 (1985).
K. M. McLean et al., "Expression In Escherichia Coli Of A Cloned Crystal Protein Gene Of Bacillus Thuringiensis Subsp. Israelensis", J. Bacteriol., 169:1017-1023 (1987).
J. T. Nishiura, "Fractionation Of Two Mosquitocidal Activities From Alkali-Solubilized Extracts Of Bacillus Thuringiensis Subspecies Israelensis Spores And Parasporal Inclusions", J. Invertebr. Pathol., 51:15-22 (1988).
M. A. Pfannenstiel et al., "Immunological Relationships Among Proteins Making Up The Bacillus Thuringiensis Subsp. Israelensis Crtystalline Toxin", Appl. Environ. Microbiol., 52:644-649 (1986).
K. Sen et al. "Cloning And Nucleotide Sequences Of The Two 130 kDA Insecticidal Protein Genes Of Bacillus Thuringiensis Var. Israelensis", Agric. Biol. Chem., 52:873-878 (1988).
W. E. Thomas et al., "Bacillus Thuringiensis Var. Israelensis Crystal .delta.-Endotoxin: Effects On Insect And Mammalian Cells In Vitro And In Vivo", J. Cell Sci., 60:181-197 (1983).
A. Undeen et al., "The Effect Of Bacillus Thuringiensis ONR60A Strain (Goldberg) On Simulium Larvae In The Laboratory", Mosq. News, 38:524-527 (1978).
B. Visser et al., "The Mosquitocidal Activity Of Bacillus Thuringiensis Var. Israelensis Is Associated With Mr 230,000 And 130,000 Crystal Proteins", FEMS Microbiol. Lett., 30:211-214 (1986).
E. S. Ward et al., "Assignment Of The .delta.-Endotoxin Gene Of Bacillus Thuringiensis Var. Israelensis To A Specific Plasmid By Curing Analysis", FEBS Lett., 158:45-49 (1983).
E. S. Ward et al., "Bacillus Thuringiensis Var. Israelensis .delta.-Endotoxin. Nucleotide Sequence And Characterization Of The Transcripts In Bacillus Thuringiensis And Escherichia Coli", J. Mol. Biol., 191:1-11 (1986).
E. S. Ward et al., "Cloning And Expression Of Two Homologous Genes Of Bacillus Thuringiensis Subsp. Israelensis Which Encode 130-Kilodalton Mosquitocidal Proteins", J. Bacteriol., 170:727-735 (1988).
E. S. Ward et al., "Cloning And Expression In Escherichia Coli Of The Insecticidal .delta.-Endotoxin Gene Of Bacillus Thuringiensis Var. Israelensis", FEBS Lett., 175:377-382 (1984).
E. S. Ward et al., "Bacillus Thuringiensis Var. Israelensis .delta.-Endotoxin. Cloning And Expression Of The Toxin In Sporogenic And Asporogenic Strains Of Bacillus Subtilis", J. Mol. Biol., 191:13-22 (1986).
D. Wu et al., "Synergism In Mosquitocidal Activity Of 26 And 65 kDA Proteins From Bacillus Thuringiensis Subsp. Israelensis Crystal", FEBS Lett., 190:232-236 (1985).
M. Young, "Gene Amplification In Bacillus Subtilis", J. Gen. Microbiol., 130:1613-1621 (1984).

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