Non-glycosylated recombinant collagen-like polypeptides

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Scleroproteins – e.g. – fibroin – elastin – silk – etc.

Reexamination Certificate

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Details

C435S440000

Reexamination Certificate

active

07932353

ABSTRACT:
The invention relates to recombinant collagen like polypeptides that are not glycosylated thereby reducing the immunogenicity of recombinant collagen-like polypeptides while at the same time preventing or reducing phosphorylation of these polypeptides. Such non-glycosylated polypeptides may be used in a wide variety of medical or cosmetic applications.

REFERENCES:
patent: 2003/0064436 (2003-04-01), Vaughan et al.
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patent: WO 01/34646 (2001-05-01), None
patent: WO 2004/075871 (2004-09-01), None
Vitagliano et al. “Structural Bases of Collagen Stabilization Induced by Proline Hydroxylation”, Biopolymers, 2001, vol. 58, pp. 459-464.
Bretthauer and Castellino, “Glycosylation of Pichia pastoris-derived proteins”, Biotechnol. Appl. Biochem (1999) 30, 193-200.
Van den Steen et al, “Concepts and Principles of O-Linked Glycosylation,” Critical Reviews in Biochemistry and Molecular Biology, 33(3): 151-208 (1988).
Felix Franks, “Long-Term Stabilization of Biologicals”, Biotechnology vol. 12 (Mar. 12, 1994).

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