Non-enzymatic methods of generating chemiluminescence from acrid

Details Non-enzymatic methods of generating chemiluminescence from acrid Non-enzymatic methods of generating chemiluminescence from acrid

1998-06-17

2000-01-25

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

435 6, 435 71, 435 26, 435 28, 435968, 436546, 436800, 436805, G01N 33532, G01N 33533, C12Q 132, C12Q 128

Patent

active

060177691

ABSTRACT:
Methods of generating chemiluminescence rapidly without the use of enzymes are provided. The methods utilize chemiluminescent compounds comprising an acridan ring bearing an exocyclic double bond in a reaction with an acid, an oxidant and a base. The chemiluminescent methods are useful in assays to detect the amount of an analyte in a sample. The chemiluminescent compounds may be supplied as a label on an analyte or a specific binding partner or may be encapsulated within a liposome or latex particle.

REFERENCES:
patent: 5521103 (1996-05-01), Zomer et al.
patent: 5656207 (1997-08-01), Woodhead et al.
patent: 5656500 (1997-08-01), Law et al.
H. Akhavan-Tafti et al, Bioluminescence and Chemiluminescence, Molecular Reporting with Photons, Proceedings of the 9th Internat'l Symp. on Biolumin. and Chemilumin. held at Woods Hole, MA, Oct. 1996, pp. 311-314, 1997.
R.Handley, H.Akhavan-Tafti, A.P. Schaap, J.Clin.Ligand Assay, 20(4) 302-312 (1997).
L.J.Kricka, Ligand-Binder Assays, Marcel Dekker, Inc., NY, 1985, pp. 15-51.
M.Z.Atassi, "Chemical Modification and Cleavage of Proteins," Ch 1 in Immuno-chemistry of Proteins, vol. 1, Plenum Press, NY, 1977, pp. 1-161.
N.Theodosiou,C.Chalot,C.Ziomek, Biotechniques, 13(6), 898-901 (1992).
H.Ji, "Bifunctional Reagents," Methods in Enzymology, 91, 580-609 (1983).
F.A.Carey, A.S.Court, J.Org.Chem., 37, 1926-29 (1972).
E.J.Corey, and A.P.Kozikowski, Tetrahedron Lett., 925-28 (1975).
U.K.Laemmli, Nature (London), 227, 680-685 (1970).

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