Non-enzymatic immunohistochemical staining system and reagents

Details Non-enzymatic immunohistochemical staining system and reagents Non-enzymatic immunohistochemical staining system and reagents

1985-08-15

1988-12-20

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 5, 435810, 424 3, 424 5, 424 71, 436501, 436512, 436519, G01N 3353

Patent

active

047925213

ABSTRACT:
A non-enzymatic method of immunohistochemical staining uses a bound electron transfer agent to achieve amplification of antibody binding to specific antigens of interest in a histology or cytology specimen. A preferred embodiment uses a bound phenazine, which is reduced with a soluble reducing agent, and which in turn reduces a tetrazolium salt, to precipitate a formazan dye at the site of primary antibody binding to the antigen of interest. Reagents and kits for use in the method are also provided.

REFERENCES:
patent: 4160645 (1979-07-01), Ullman
patent: 4556634 (1985-12-01), Misaki et al.
patent: 4598042 (1986-07-01), Self
patent: 4654300 (1987-03-01), Zuk et al.
Weiss, L., Histology Cell and Tissue Biology, (fifth edition), Elsevier Science Publishing Co., New York, pp. 98-108, (1983).

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