Non-desensitizing mutant of the transient receptor potential...

Details Non-desensitizing mutant of the transient receptor potential... Non-desensitizing mutant of the transient receptor potential...

2011-01-25

2011-01-25

Saoud, Christine J (Department: 1647)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S069100, C435S252300, C435S320100, C536S023500

Reexamination Certificate

active

07875452

ABSTRACT:
There exists a need in the art for high throughput screening assays that can identify compounds that specifically modulate the activity of fast-acting ion channels, such as TRPM5. Current methods, especially electrophysiological, suffer from a lack of sensitivity, rapid signal loss, low throughput, and are labor intensive. The claimed methods and compositions provide electrophysiology methods that allow prolonged sample testing and fluorescent assays with an optical readout that gives rapid readout of the results, has a high signal to noise background ratio, are easy to use, can be modified for automation and miniaturization, and provide verification that a compound specifically modulates TRPM5.

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