Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1991-02-01
1992-07-14
Wax, Robert A.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
935 19, 935 20, 935 21, 436175, 436177, C07H 2100
Patent
active
051304236
ABSTRACT:
This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase. Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction. The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
REFERENCES:
patent: 4440778 (1984-04-01), Matsui et al.
Kirby, K. S. (1956) "A New Method for the Isolation of Ribonucleic Acids from Mammalian Tissues", Biochem. J. 64 405-408.
Kirby, K. S. (1957) "A New Method for the Isolation of Deoxyribonucleic Acids: Evidence on the Nature of Bonds between Deoxyribonucleic Acid & Protein", Biochem. J 66:495-504.
Cimler B. Melina
Meyer, Jr. Rich B.
Ness Jeffrey Van
Vermeulen Nicolaas M. J.
Bugaisky G.
Leith Debra
Microprobe Corporation
Wax Robert A.
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