Chemistry: molecular biology and microbiology – Vector – per se
Patent
1998-05-12
1999-12-21
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Vector, per se
435456, 435463, 536 231, C12N 1563, C12N 1500, C12N 1511
Patent
active
060048042
ABSTRACT:
The invention is based on the discovery that Duplex Mutational Vectors are active in prokaryotic cells that contain a strand transfer activity (RecA) and mismatch repair activity (MutS). Using this system a type of Duplex Mutational Vector, termed a Non-Chimeric Mutational Vector, having no RNA:DNA hybrid-duplex, was shown to be active in prokaryotic cells if protected from 3' exonuclease attack. Such protection can be conferred by replacement of a tetrathymidine linker by a nuclease resistant oligonucleotide, such as tetra-2'-O-methyl-uridine, to link the two strands of the recombinagenic oligonucleobase. The claims concern 3'-exonuclease protected Non-Chimeric Mutational Vectors.
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Kumar Ramesh
Metz Richard A.
Kimeragen, Inc.
LeGuyader John L.
Shibuya Mark L.
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