Non-centrifugation method for immunoassay of materials

Chemistry: analytical and immunological testing – Thyroid hormone tests

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436510, 436538, 436541, 436800, 436801, 436804, 436809, 436810, 436817, 436824, 436808, G01N 3358, G01N 3500, G01T 100

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044566905

ABSTRACT:
The present invention relates to a method which eliminates centrifugation and decantation steps, to be performed in an automatic manner for carrying out specific binding assay tests, wherein liquid and solid phases are present.
According to the invention, use is made of a specially designed device, consisting of a mixing reservoir into which is fitted snugly a mixer separator having a channel in the vertical axis of the mixer-separator. A rack holding a number of said mixing reservoirs containing the incubated reagents and analytes, capped with the mixer separators, is placed into a press-device designed to perform at a controlled rate a downward movement. The mixer separators are pushed downwards into the mixing reservoirs at a chosen rate for a preselected distance to complete the mass transport and separation operations. The separation devices are removed and either one of the separated phases can be measured in the desired analytical instrument for a quantitative or qualitative determination.
The results obtained according to the present invention compare very favorably with those determined by other methods requiring centrifugation and/or decantation in the assay protocol.

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patent: 4235865 (1980-11-01), Thoma
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