Non-aqueous solvent specific binding protein assays

Details Non-aqueous solvent specific binding protein assays Non-aqueous solvent specific binding protein assays

1990-10-31

1992-06-02

Nucker, Christine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 795, 435 794, 435 793, 435 792, 436501, 436518, 436527, C12Q 100, G01N 3353, G01N 33566, G01N 33543

Patent

active

051186071

ABSTRACT:
Novel specific protein binding assays are provided employing a solvent system comprising a solvent characterized as hydrophobic, essentially anhydrous and water immiscible. Particularly, lipophilic analytes are shown to be sensitively determined using antibodies in hydrocarbon media, optionally substantially saturated with aqueous buffer and/or comprising an anionic surfactant.

REFERENCES:
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The Merck Index (1983), pp. 1073, 970, The Merck & Co, Rahway N.J.
Tinoco et al. Physical Chemistrty. Prentice-Hall, N.J., 1987, pp. 85-90.
Kabanov et al., A New Way in Homogeneous Immunoassay: Reversed Micellar Systems as a Medium for Analysis, Analyt. Biochem 181:145-8, 1989.
Zaks et al, Enzyme Catalysis in Non-Aqueous Sovents. J. Biol. Chem 263(7):3194-3201 Mar. 1988.
Russell et al. Antibody-Antigen Binding in Organic Solvents. Biochem Biophys Res Com 158(1) 80-5 Jan. 89.

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