Non-antibiotic selection marker genes

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

Reexamination Certificate

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C536S023600, C435S195000, C435S320100, C435S431000, C435S468000

Reexamination Certificate

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07629502

ABSTRACT:
The present invention provides for a DNA construct comprising a promoter, a TSB1 gene, and a genetic modification, wherein the TSB1 gene and the genetic modification are promoted by the same said promoter. Additionally, the present invention provides for a plant that is transfected by this DNA construct, and a method of selecting this transfected plant.

REFERENCES:
patent: 5278057 (1994-01-01), Jorgensen
patent: 7045678 (2006-05-01), Chiu
patent: 2002/0061570 (2002-05-01), Anderson et al.
patent: 2002/0168709 (2002-11-01), Hennecke et al.
Last, et al., (1991). Tryptophan mutants inArabidopsis: the consequences of duplicated tryptophan synthase beta genes. Plant Cell 3: 345-358.
Barczak et al, 5-Fluoroindole resistance identifies tryptophan synthase beta subunit mutants inArabidopsis thaliana. Genetics, (May 1995) vol. 140, No. 1, pp. 303-313.
Weber-Ban, Investigation of allosteric linkages in the regulation of tryptophan synthase: the roles of salt bridges and monovalent cations probed by site-directed mutation, optical spectroscopy, and kinetics. Biochemistry, (Mar. 27, 2001) vol. 40, No. 12, pp. 3497-3511.
Hyde, et al, 1988, Three-dimensional structure of the tryptophan synthase α2β2 multienzyme complex fromSalmonella typhimuriumJ. Biol. Chem. 263: 17857-17871.
www.mcb.arizona.edu/tax/2010/vector.cfm?id=pBIN 19.
Avonce, N., Leyman, B., Mascorro-Gallardo, J.O., Van Dijck, P., Thevelein, J.M., and Iturriaga, G (2004). TheArabidopsis trehalose-6-P synthase AtTPS1 gene is a regulator of glucose, abscisic acid, and stress signaling.Plant Physiol136, 3649-3659.
Breitler, J.C., Meynard, D., Van Boxtel, J., Royer, M., Bonnot, F., Cambiliau, L., and Guiderdoni, E. (2004). A novel two T-DNA binary vector allows efficient generation of marker-free transgenic plants in three elite cultivars of rice (Oryza sativaL.).Transgenic Res13, 27 1-287.
Chan YL, Lin KH, Sanjaya, Liao LJ, Chen WH, Chan MT (2005). Gene stacking inPhalaenopsisorchid enhances dual tolerance to pathogen attack.Transgenic Res14:279-288.
Cho, H.J., Brotherton, J.E., and Widholm, J.M. (2004). Use of the tobacco feedback-insensitive anthranilate synthase gene (ASA2) as a selectable marker for legume hairy root transformation.Plant Cell Rep23, 104-113.
Cho, H.J., Brotherton, J.E., Song, H.S., and Widholm, J.M. (2000). Increasing tryptophan synthesis in a forage legumeAstragalus sinicusby expressing the tobacco feedback-insensitive anthranilate synthase (ASA2) gene. Plant Physiol 123, 1069-1076.
Clough, S.J., and Bent, A.F. (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation ofArabidopsis thaliana. Plant J16, 735- 743.
Daniell H, Muthukumar B, Lee SB (2001a) Marker free transgenic plants: engineering the chloroplast genome without the us of antibiotic selection.Curr Genet39:109-116.
Daniell H, Wiebe PO, Millan AF (2001a) Antibiotic-free chloroplast genetic engineering—an environmentally friendly approach.Trends Plant Sci6:237-239.
de Vetten, N., Wolters, A.M., Raemakers, K., van der Meer, I., ter Stege, R., Heeres, E., Heeres, P., and Visser, R. (2003). A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop.Nat Biotechnol21, 439-442.
Ebmeier, A., Allison, L., Cerutti, H., and Clemente, T. (2004). Evaluation of theEscherichia colithreonine deaminase gene as a selectable marker for plant transformation.Planta218, 751-758.
Edlund, A., Ekiof, S., Sundberg, B., Moritz, T., and Sandberg, G (1995). A Microscale Technique for Gas Chromatography—Mass Spectrometry Measurements of Picogram Amounts of Indole-3-Acetic Acid in Plant Tissues.Plant Physiol108, 1043-1047.
Erikson, 0., Hertzberg, M., and Nasholm, T. (2004). A conditional marker gene allowing both positive and negative selection in plants.Nat Biotechnol22, 455-458.
Erikson, 0., Hertzberg, M., and Nashoim, T. (2005). The dsdA gene fromEscherichia coliprovides a novel selectable marker for plant transformation.Plant Mol Biol57, 425-433.
Haldrup, A., Petersen, S., and Okkels, F. (1998a). Plant xylose isomerase gene fromThermoanaerobacterium thermosulfurogenesallows efective selection of transgenic plant cells using D-xylose as the selection agent.Plant Mol Biol37, 287-296.
Haldrup, A., Petersen, S.G, and Okkels, F.T. (1998b). Positive selection: a plant selection principle based on xylose isomerase, an enzyme used in the food industry.Plant Cell Rep18:76-81.
Hsieh, T.H., Lee, J.T., Charng. Y.Y., and Chan, M.T. (2002a). Tomato plants ectopically expressingArabidopsisCBF 1 show enhanced resistance to water deficit stress.Plant Physiol130, 618-626.
Hsieh, T.H., Lee, J.T., Yang, P.T., Chiu, L.H., Charng, Y.Y., Wang, Y.C., and Chan, M.T. (2002b). Heterology expression of theArabidopsisC-repeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato.Plant Physiol129, 1086-1094.
Hull, A.K., Vij, R., and Celenza, J.L. (2000).Arabidopsiscytochrome P450s that catalyze the first step of tryptophan-dependent indole-3-acetic acid biosynthesis.Proc Natl Acad SciU S A 97, 2379-2384.
Joersbo, M. (2001). Advances in the selection of transgenic plants using non-antibiotic marker genes.Physiologia Plantarum111, 269-272.
Kanno, T., Kasai, K., Ikejiri-Kanno, V., Wakasa, K., and Tozawa, V. (2004). In vitro reconstitution of rice anthranilate synthase: distinct functional properties of the alpha subunits OASAI and OASA2.Plant Mol Biol54, 11-22.
Kisaka H, Kisaka M, Lee HY, Kmeya T (1998) Isolation of a cDNA for tryptophan synthase β from rice and studies of its expression in a 5-methyltryptophan-resistant mutant of rice.Plant Mol Biol38:875-878.
Last, R.L., Bissinger, P.H., Mahoney, D.J., Radwanski, E.R., and Fink, GR. (1991). Tryptophan mutants inArabidopsis: the consequences of duplicated tryptophan synthase beta genes.Plant Cell3, 345-358.
Leyman, B., Avonce, N., Ramon, M., Van Dijck, P., Thevelein, J.M., and Iturriaga, G- (2004). New selection marker for plant transformation.Methods Mol Biol267, 385-396.
Leyman, B., Avonce, N., Ramon, M., Di] ck, P.V., Iturriaga, G, and Thevelein, J.M. (2005). Trehalose-6-phosphate synthase as an intrinsic selection marker for plant transformation.J Biotechnol.
Liao, L.-J., Pan, I.C., Chan, Y.-L., Hsu, Y.-H., Chen, W.-H., and Chan, M.-T. (2004). Transgene silencing inPhalaenopsisexpressing the coat protein of Cymbidium Mosaic Virus is a manifestation of RNA-mediated resistance.Molecular Breeding13:229-242.
Mentewab A, Stewart CN Jr (2005) Overexpression of anArabidopsis thalianaABC trasporter confes kanamycin resistance to transgenic plants.Nat Biotechnol23:1177-1180.
Miki, B., and McHugh, 5. (2004). Selectable marker genes in transgenic plants: applications, alternatives and biosafety. J Biotechnol 107:193-232.
Pomponi M, Censi V, Di Girolamo V, De Paolis A, di Toppi LS, Aromolo R, Costantino P, Cardarelli M (2006) Overexpression ofArabidopsis phytochelatinsynthase in tobacco plants enhances Cd(2+) tolerance and accumulation but not translocation to the shoot.Planta223:180-190.
Pruitt, K.D., and Last, R.L. (1993). Expression patterns of duplicate tryptophan synthase beta genes inArabidopsis thaliana. Plant Physiol102, 10 19-1026.
Taulavuori, K., Prasad, M.N., Taulavuori, E., and Lame, K. (2005). Metal stress consequences on frost hardiness of plants at northern high latitudes: a review and hypothesis.Environ Pollut135, 209-220.
Tian L, Jordan M, Miki B (2006) Markers ad selector genes for plant transformation. In: Jaime A Teixeira da Silva (eds) Ornamental an plant biotechnology, vol. II.Global Science Books, London, pp. 9-20.
Tian L (2006) Markers gene removal from transgenic plants. In: Jaime A Teixeira da Silva (eds) Ornamental and plant biotechnology, vol. II.Global Science Books, London, pp. 26-29.
Tozawa, Y., Hasegawa, H., Terakawa, T., and Wakasa, K. (2001). Characterization of rice anthranilate synthase alpha-subunit genes OASAI and OASA2. Tryptophan accumulation in transgenic rice expressing a feedback-insensitive mutant of OASAI.Plant Physiol126, 1493-1506.
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