Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal
Reexamination Certificate
2006-11-03
2010-12-28
Long, Scott (Department: 1633)
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
Transgenic nonhuman animal
C800S014000, C800S018000, C800S295000
Reexamination Certificate
active
07858844
ABSTRACT:
Nucleic acid compositions encoding non-aggregating chromo/fluoroproteins and mutants thereof, as well as the proteins encoded by the same, are provided. The proteins of interest are polypeptides that are non-aggregating colored and/or fluorescent proteins, where the non-aggregating feature arises from the modulation of residues in the N-terminus of the protein and the chromo and/or fluorescent feature arises from the interaction of two or more residues of the protein. Also provided are fragments of the subject nucleic acids and the peptides encoded thereby, as well as antibodies to the subject proteins and transgenic cells and organisms. The subject protein and nucleic acid compositions find use in a variety of different applications. Finally, kits for use in such applications, e.g., that include the subject nucleic acid compositions, are provided.
REFERENCES:
patent: 5491084 (1996-02-01), Chalfie et al.
patent: 5874304 (1999-02-01), Zolotukhin et al.
patent: 5919445 (1999-07-01), Chao
patent: 5958713 (1999-09-01), Thastrup et al.
patent: 5968738 (1999-10-01), Anderson et al.
patent: 5968750 (1999-10-01), Zolotukhin et al.
patent: 5976796 (1999-11-01), Szalay et al.
patent: 5985577 (1999-11-01), Bulinski
patent: 6020192 (2000-02-01), Muzyczka et al.
patent: 6066476 (2000-05-01), Tsien et al.
patent: 6342379 (2002-01-01), Tsien et al.
patent: 6969597 (2005-11-01), Lukyanov et al.
patent: 7166444 (2007-01-01), Lukyanov et al.
patent: 2003/0186229 (2003-10-01), Tsien et al.
patent: 19718640 (1999-07-01), None
patent: WO 99/49019 (1999-09-01), None
patent: WO 00/46233 (2000-02-01), None
patent: WO 00/34318 (2000-06-01), None
patent: WO 00/34319 (2000-06-01), None
patent: WO 00/34320 (2000-06-01), None
patent: WO 00/34321 (2000-06-01), None
patent: WO 00/34322 (2000-06-01), None
patent: WO 00/34323 (2000-06-01), None
patent: WO 00/34324 (2000-06-01), None
patent: WO 00/34325 (2000-06-01), None
patent: WO 00/34326 (2000-06-01), None
patent: WO 01/27150 (2001-04-01), None
Kappel et al (1992) Current Opinion in Biotechnology 3, 548-553.
Mullins et al (1993) Hypertension 22, p. 630-633.
Houdebine (1994) J. Biotech. 34, p. 269-287.
Mullins et al. (J. Clin. Invest.1996; 98, p. 1557-1560.
Cameron (1997) Molec. Biol. 7, p. 253-265.
Sigmund (2000) Arteroscler. Throm. Vasc. Biol. 20, p. 1425-1429.
Niemann (1998) Transg. Res. 7, p. 73-75.
Moreadith et al., J. Mol. Med., 1997, p. 208-216.
Pera et al. [Journal of Cell Science 113: 5-10 (2000)].
Wall (1996) Theriogenology 45, 57-68.
Protein Sequence Similarity—SEQ13 & Tsien6.
Harper et al (Nature Biotechnology. Nov. 1999; 17: 1125-1129).
Baird et al. (PNAS. Oct. 24, 2000; 97(2): 11984-11989).
Patterson et al. (Biophysical Journal. Nov. 1997; 73: 2782-2790).
Wall et al. (Nature Structural Biology. Dec. 2000; 7(12): 1133-1136).
Protein Sequence Similarity—SEQ13 & Tsien (Mar. 16, 2009).
Anderluh et al. “Cloning, Sequencing, and Expression of Equinatoxin II,”Biochem. Biophys. Res. Comm.(1996) 220: 437-442.
Dove et al. “Isolation and Partial Characterization of the Pink and Blue Pigments of Pocilloporid,”Biol. Bull.(1995) 189: 288-297.
Fradkov et al. “Novel Fluorescent Protein From Discosoma Coral and its Mutants Possesses a Unique Far-Red Fluorescence,”FEBS Letters(2000) 479: 127-130.
Gurskaya et al. “GFP-Like Chromoproteins as a Source of Far-Red Fluorescent Proteins,”FEBS Letters(2001) 507: 16-20.
Gurskaya et al. “Color Transitions in Coral's Fluorescent Proteins by Site-Directed Mutagenesis,”BMC biochemistry(2001) 2:6.
Lukyanov et al. “Natural Animal Coloration can be Determined by a Nonfluorescent Green Fluorescent Protein Homolog,”The Journal of Biological Chemistry(2000) 275 (34): 25879-25882.
Ma{hacek over (c)}ek et al. “Intrinsic Tryptophan Fluorescence of Equinatoxin II, a Pore-Forming Polypeptide from the Sea AnemoneActinia equinaL, Monitors its Interaction with Lipid Membranes,”Eur. J. Biochem.(1995) 234: 329-335.
Martynov, et al. “Alternative Cyclization in GFP-Like Proteins Family,”Journal of Biological Chemistry(2001) 276(24): 21012-21016.
Matz et al. “Fluorescent Proteins from Nonbioluminescent Anthozoa Species,”Nature Biotechnology(1999) 17: 969-973.
Terskikh et al. “‘Fluorescent Timer’: Protein that Changes Color with Time”,Science(2000) 290:1585-1588.
Tsein “The Green Fluorescent Protein,”Ann. Rev. Biochem.(1998) 67: 509-544.
Tsein “Rosy Dawn for Fluorescent Proteins,”Nature Biotechnology(1999 ) 17: 956-957.
Ward et al. “An Energy Transfer Protein in Coelenterate Bioluminescence,”J. Bio. Chem.(1979) 254(4): 781-787.
Wiedenmann et al. “The Ectodermal Pigments of the Morphs ofAnemoniasp. (sulcata) (Pennant)(Cnidaria; Anthozoa): An Adaptation to the Life in Different Depths,”Molecular Biology(2000) 36(4): 521-528.
Yanushevich et al. “A Strategy for the Generation on Non-Aggregating Mutants ofAnthozoaFluorescent Proteins,”FEBS(2002) 511: 11-14.
Yarbrough et al. “Refined Crystal Structure of DsRed, a Fluorescent Protein from Coral, at 2.0-Å Resolution,”Proc. Natl. Acad. Sci(2001) 98(2): 462-467.
R. Wetzel “Mutations and Off-Pathway Aggregation of Proteins,”Tibtech(1994) 12:193-198.
G. Baird et al. “Biochemistry, Mutagenesis, and Oligometrization of DsRed, a Red Fluorescent Protein from Coral,”Proc. Natl. Acad. Sci(2000) 97(22):11984-11989.
Wall et al. “The Structural Basis for Red Fluorescence in the Tetrameric GFP Homolog DsRed,”Nature Structural Biology(2000) 7(12):1133-1138.
Wiehler et al. “Mutants of Discosoma Red Fluorescent Protein with a GFP-Like Chromophore,”FEBSLetter (2001) 487:384-389.
Wells, Biochemistry, (1990) 29:8509-8517.
Heim et al. Proc. Natl. Acad. Sci (1994) 91:12501-12504.
Lund et al. Biophysical Journal (2003) 85(5):2940-2947.
Matz M.V. et al, “Flourescent Proteins from Nonbioluminescent Anthozoa Species” Nature Biotechnology, Oct. 1999, 17:969-973.
Database Nucleotide [Online] Oct. 21, 1999, “Discosoma sp. fluorescent protein FP583 mRNA, complete cds” retrieved from NCBI Database accession No. AF168419.
Database Nucleotide [Online] Oct. 21, 1999, “Discosoma sp. Fluorescent protein FP483 mRNA, complete cds” retrieved from NCBI Database accession No. AF168420.
Database Nucleotide [Online] Oct. 21, 1999, “Discosoma sp. fluorescent protein FP486 mRNA, complete cds” retrieved from NCBI Database accession No. AF168421.
Database Nucleotide (online) “Anemonia sulcata green fluorescent protein as FP499 mRNA, complete cds” retrieved from NCBI Database accession No. AF322221.1 Nov. 19, 2000.
Database Nucleotide (online) “Anemonia sulcata nonfluorescent red protein as CP562 mRNA, complete cds” retrieved from NCBI Database accession No. AF322222.2 Feb. 28, 2002.
Mazel: “Spectral measurements of fluorescence emissions”; Marine Ecology Progress Series, vol. 120, Apr. 20, 1995 pp. 185-191.
Prasher et al., Primary structure of the Aequorea victoria green fluorescent protein: GENE vol. 111, (1992), pp. 229-233.
Wiedenmann, J., “Zur Biologie der Okoypen von Annemonia sulcata (Pennant)” DIPLOMARBEIT, (1996) Ulm.
Bokman et al., “Renaturation of Aequorea green-fluorescent protein” Biochemical and Biophysical Research Communications; vol. 101, No. 4 (1981) pp. 1372-1380.
Cubitt et al., “Understanding, improving and using green fluorescent proteins” Trends in Biochemical Sciences, vol. 20, No. 11 (1995) pp. 448-455.
Ward et al., “Spectrophotometric Identity of the Energy Transfer Chromophores in Renilla and Aequorea Green-Fluorescent Proteins”; Photochemistry and Photobiology, vol. 31, No. 6; (1980) pp. 611-616.
Fradkov Arcady
Lukyanov Konstantin
Lukyanov Sergey
Savitsky Alexandr
Yanushevich Yuriy
Bozicevic Field & Francis LLP
Clontech Laboratories, Inc.
Field Bret E.
Long Scott
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